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First Color Images Produced By an Electron Microscope (sciencemag.org)

Slashdot reader sciencehabit quotes Science magazine: Imagine spending your whole life seeing the world in black and white, and then seeing a vase of roses in full color for the first time. That's kind of what it was like for the scientists who have taken the first multicolor images of cells using an electron microscope. Electron microscopes can magnify an object up to 10 million times, allowing researchers to peer into the inner workings of, say, a cell or a fly's eye, but until now they've only been able to see in black and white. The new advance -- 15 years in the making -- uses three different kinds of rare earth metals called lanthanides...layered one-by-one over cells on a microscope slide. The microscope detects when each metal loses electrons and records each unique loss as an artificial color.

46 comments

  1. "rare earth metals called lanthanides"? by K.+S.+Kyosuke · · Score: 2

    Ugh, that is awful phrasing. Almost like "three different kinds of cars called automobiles".

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    Ezekiel 23:20
    1. Re:"rare earth metals called lanthanides"? by Anonymous Coward · · Score: 0

      Ugh, that is awful phrasing. Almost like "three different kinds of cars called automobiles".

      And each automobile-car with a unique, artificial color too! That'll sell.

    2. Re:"rare earth metals called lanthanides"? by ArmoredDragon · · Score: 4, Insightful

      The article itself is full of fail in that regard. They literally call it a color image when it's psuedocolor, just like we've always gotten out of SEM images, the only thing is that the methodology is different in this case.

    3. Re:"rare earth metals called lanthanides"? by Anonymous Coward · · Score: 0

      No, it's not. It's the exact opposite.

      It's:
      Rare earth metals:lanthanides::automobiles:cars

      It's *not*, what you suggest:
      Rare earth metals:lanthanides::cars:automobiles

      Your analogy should be "three different kinds of automobiles called 'cars'". Which is a perfectly fine thing to say. It only sounds abnormal because everyone knows what a car is.

    4. Re:"rare earth metals called lanthanides"? by gb · · Score: 1

      I think technically the analogy would be "three different kings of road vehicles called automobiles" (you might think the actinides also counted as rare earths) - but the substantive point that the language is clunky still holds.

    5. Re: "rare earth metals called lanthanides"? by Anonymous Coward · · Score: 0

      Rare earths and lanthanides are the same thing. Those terms refer to the same section of the periodic table. I agree, it's awful phrasing. Why not just say it's lanthanum, cerium, and praseodymium? It's definitely a false color image, too. The electrons don't have a color, so it's definitely not going to show real colors. Instead, the different metals only stick to certain types of molecules, so you're differentiating structures based on the types of molecules in them and the metals that stick in those locations.

    6. Re:"rare earth metals called lanthanides"? by K.+S.+Kyosuke · · Score: 1

      Actinides have anything to do with rare earths? In that case, I was lied to in school!

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      Ezekiel 23:20
    7. Re:"rare earth metals called lanthanides"? by K.+S.+Kyosuke · · Score: 1

      Clearly I'm not familiar with American car-automobile-speak. My point was that one of the things referenced in the summary is more or less the same thing as the other by definition.

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      Ezekiel 23:20
    8. Re:"rare earth metals called lanthanides"? by gb · · Score: 1

      It's semantics really. IUPAC woul say that they are the lanthanides plus yttrium and scandium. I've encountered the term being used more generally (i.e. by non-chemists) to mean anything that's 'down-there' on the periodic table.

    9. Re:"rare earth metals called lanthanides"? by K.+S.+Kyosuke · · Score: 1

      It's not "semantics" (although it is actual linguistic semantics, of course - but then again, that's too broad to be useful when debating meanings of utterances) if you do or do not include a whole new category of elements, most of them synthetic and not found in nature at all.

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      Ezekiel 23:20
    10. Re:"rare earth metals called lanthanides"? by ChrisMaple · · Score: 1

      Rare earths include scandium and yttrium in addition to the lanthanides.
      Car and automobile are used interchangeably, but... car can also refer to a railroad car, and by stretching common usage a bit automobile refers to all self propelled land vehicles large enough to carry a person. Although if you refer to a motorcycle or a garbage truck as an automobile, you'll only confuse people.

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    11. Re:"rare earth metals called lanthanides"? by K.+S.+Kyosuke · · Score: 1

      Yes, Sc, and Y are commonly included in the group for various, mostly historical reasons. Regarding the road vehicle terminology, it's probably interference from my native tongue where the two equivalent terms actually mean basically the same thing. I could/should have made a different analogy instead.

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      Ezekiel 23:20
    12. Re:"rare earth metals called lanthanides"? by Anonymous Coward · · Score: 0

      No. In addition to "cars", "automobiles" include "trucks", etc.

    13. Re:"rare earth metals called lanthanides"? by K.+S.+Kyosuke · · Score: 0

      The summary didn't mention either cars or automobiles, if you care to reread it.

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      Ezekiel 23:20
    14. Re:"rare earth metals called lanthanides"? by Dogtanian · · Score: 1

      The article itself is full of fail

      Indeed it is.

      I read it wondering how- and if- this process was supposed to produce literal colour images. While I figured out that it probably wasn't, I'm still left wondering how this process is supposed to help is see things better, and what the (pseudo) colours produced by it are supposed to represent- because the article certainly doesn't explain.

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    15. Re: "rare earth metals called lanthanides"? by Dogtanian · · Score: 1

      Instead, the different metals only stick to certain types of molecules, so you're differentiating structures based on the types of molecules in them and the metals that stick in those locations.

      Ah, thanks. Assuming that's correct, it's odd that you seemed able to explain that in a straightforward manner, yet the alleged article couldn't. :-/

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  2. I call BS by Anonymous Coward · · Score: 1

    There is no color at that scale, that's false color, and we've seen colorized images plenty...

    If they really want to show some "color" then shift the spectrum of the feature size and map it to visible light, that will at least be proportional color reproduction instead of a few arbitrary dots in a grayscale image.

    captcah is "COMPOST" which is what the article claim is

    1. Re:I call BS by dak664 · · Score: 1

      That would be one way, although false coloring through a full spectrum shift seems not to work as well as limited black body color temperature scales. But the potential at every voxel can be derived from the electron scattering, and the gradient of that potential could give a true color image without any shifting. Don't know if any of the colors would be visible to humans, though.

  3. Psuedocolor please... by The+Real+Dr+John · · Score: 4, Informative

    This is cool, but not color electron microscopy. It is pseudocolor at best based on what things in a cell that the lanthanides bind to. A real color electron microscope would somehow use electrons at different energies to try and figure out the chemical makeup of subcellular structures. Or maybe vaporize the sample line by line by scanning with the electron beam on high after taking the image with lower energy electrons, and then analyze the ions produced. But this is still pretty cool.

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    1. Re:Psuedocolor please... by K.+S.+Kyosuke · · Score: 1

      I was also kind of expecting that they found out how to make electrons figure out the actual colors. But this seems to be an intractable problem, physics-wise. Sadly.

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      Ezekiel 23:20
    2. Re:Psuedocolor please... by Anonymous Coward · · Score: 0

      Vaporizing the sample to look at it? That's one of the dumbest things I've ever heard.

    3. Re:Psuedocolor please... by K.+S.+Kyosuke · · Score: 1

      It's problematic because colors can be determined by properties of molecules than don't exist in a cloud of ions anymore. Therefore trying to infer colors of molecular material from a cloud of elemental ions is a lost cause to begin with.

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      Ezekiel 23:20
    4. Re:Psuedocolor please... by K.+S.+Kyosuke · · Score: 1

      That should have read "by properties of molecules, which don't exist in a cloud of ions anymore". Late night hour and language barrier strikes again. Sorry for the confusion.

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      Ezekiel 23:20
    5. Re:Psuedocolor please... by Tablizer · · Score: 1

      A real color electron microscope...vaporize the sample line by line...then analyze the ions produced.

      "Hey, you found a really unique specimen here! I see a big research paper. Let's revisit that sample."

      You: "Uhhhh..."

  4. Electron Color by Anonymous Coward · · Score: 0

    I wasn't aware electrons came in different colors.

    1. Re:Electron Color by ChrisMaple · · Score: 1

      It has to do with the flavor of quarks.

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    2. Re: Electron Color by Anonymous Coward · · Score: 0

      Colours? Flavours? This is starting to sound like condoms. Do they come in ribbed and dotted too?

  5. ...and the news is....? by gb · · Score: 4, Informative

    This doesn't seem such a huge advance - we've been making elemental maps in STEMs for ages and combining several in false colour images for like also ages. As far as I can tell the new thing is that they're doing this by actually imaging selected portions of an electron energy loss spectra (rather than just recording the spectra point-wise) - I guess this makes it slightly faster to generate the image - but there's not really any new science in this.

    1. Re:...and the news is....? by Anonymous Coward · · Score: 0

      generally visualizing the sample this way is destructive to the sample (CryoEM is generally limited in its resolution by this dose limit), therefore you dont actually get to image the same thing multiple times. This way, when you are taking that single image, and based on the energy loss of the electron, figure out what part is covered in what.

  6. First Image avaiable by rsilvergun · · Score: 0

    right here.

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  7. Breakthrough by Anonymous Coward · · Score: 0, Troll

    Now we can finally see what Trumps micropenis looks like.

    1. Re: Breakthrough by Anonymous Coward · · Score: 0

      A real-life nullity?

      Finally the proof we need!

  8. Electrons don't come in colors by Lisandro · · Score: 5, Insightful

    The title is misleading - this is false color, but one generated by the electron microscope itself instead of postprocessing. As the article states, it allows for much better contrast than grayscale.

  9. Big by JustOK · · Score: 1

    Big whoop. Cellies.

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    rewriting history since 2109
  10. black and white? by Anonymous Coward · · Score: 0

    Electron microscope images are gray scale.
    Also, features much smaller than the wavelength of light will not reflect specific dominant wavelengths. I.e. color. This is a method for sudocoloringof images.

  11. That's quaint! by Anonymous Coward · · Score: 1

    Transmission electron energy loss microscopy is well established technique. I guess it's neat they did it with cells, I suppose it's kinda hard to do in high vacuum without destroying the cell; but material science has been downing this for a while... I suppose it's typical for when biology uses a well established technique used in the physical sciences to make a big deal out of it. Hopefully, they'll make some cell biology discoveries using this instead of more technique papers...

    Obviously it has to be a pseudo-color plot corresponding to the energy loss, the detected electrons don't have visible color...

  12. oh, biologists... by Goldsmith · · Score: 2

    Electron Energy Loss Spectroscopy is pretty old
    https://en.wikipedia.org/wiki/...

    The TEM version is a bit newer. It's only 20 years old.

    1. Re:oh, biologists... by Anonymous Coward · · Score: 0

      This is _all_ old Tech. X-Ray and Gamma Ray, (The difference is mainly Historical, they are all just Photons after all...), Microscopy is the future, and it is inherently Spectroscopic in nature. Unfortunately, the Light Sources and Detectors needed are still large, rare, and expensive.
      https://en.wikipedia.org/wiki/Synchrotron_light_source

  13. Adjustment Bureau by Anonymous Coward · · Score: 0

    election microscope?

  14. Probably Nancy Pelosi's idea by raymorris · · Score: 1

    > Vaporizing the sample to look at it? That's one of the dumbest things I've ever heard.

    It's kinda like "we have to pass the bill to find out what's in it."

  15. IANAP, but by aussersterne · · Score: 1

    given the properties of light photos, the nature of color, and the relationships between color as a perceptual phenomenon, photons, and objects of this scale, I would have imagined that "color" (as in natural color, i.e. color in the conventional sense and its relationship to perception and human anatomy) is not a terribly meaningful of important concept at this scale. Am I wrong?

    This is color used as an unrelated tool—applying color to enhance, essentially, actuance. Yes?

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    1. Re:IANAP, but by mlyle · · Score: 1

      I think the concept is similar to most color high magnification microscopy--- you stain things, and the stuff that's stained is a different color in the picture. From this you get additional contrast and information.

    2. Re:IANAP, but by RockDoctor · · Score: 1

      I think the concept is similar to most color high magnification microscopy

      I'm a geologist, you insensitive clod!

      The interference colours we see under crossed polarisers are real colours, intrinsic to the material, it's orientation and the radiation impacting it. (Also, pleochroism visible under polarised illumination without the analyser in the optical assembly.) No stains involved. (Though we do sometimes use stains to improve contrast too, e.g. Aliziarin Red to differentiate between different carbonate minerals.)

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  16. there's a Simpsons episode for that by Anonymous Coward · · Score: 0

    Benjamin: Come on, Mr. Simpson, you'll never pass this course without learning the periodic table.
    Homer: I'll write it on my hand.
    Benjamin: Hoh! Including all known lanthanides & actinides? Good luck!

  17. Bullshit by Anonymous Coward · · Score: 0

    Electron microscopes can only image conductors. To view a cell you have to coat the cell, an ant, etc in a thin layer of metal via deposition. There is only one color to see because you're taking reflections off a metal, not the object being studied.