Why is the use of Fisher's test on a 2x2 contingency table incorrect? Any why do you feel the two-tailed test would be more appropriate than the one-sided one? You presented no argument in either case.
Really, why your post was modded +5 insightful when it lacks any explanations at all is a mystery to me. Oh wait, hang on, this is Slashdot, where out-of-hand rejections to analyses are uncritically accepted (particularly knee-jerk reactions to the use of statistics), so long as they conform to everyone's preconceived ideas and opinions...
The final enzyme did work with real HIV in the lab. They identified a site in HIV similar to the cre binding motif, but which cre was not able to bind. They created intermediate sequences to bridge the gap between the cre binding site and this HIV sequence. Using directed evolution they could evolve cre to bind sites progressively more unlike the cre site and progressively more like the HIV site. The final outcome was an enzyme able to excise sequences flanked by the HIV specific pattern.
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Why is the use of Fisher's test on a 2x2 contingency table incorrect? Any why do you feel the two-tailed test would be more appropriate than the one-sided one? You presented no argument in either case.
Really, why your post was modded +5 insightful when it lacks any explanations at all is a mystery to me. Oh wait, hang on, this is Slashdot, where out-of-hand rejections to analyses are uncritically accepted (particularly knee-jerk reactions to the use of statistics), so long as they conform to everyone's preconceived ideas and opinions...
The final enzyme did work with real HIV in the lab. They identified a site in HIV similar to the cre binding motif, but which cre was not able to bind. They created intermediate sequences to bridge the gap between the cre binding site and this HIV sequence. Using directed evolution they could evolve cre to bind sites progressively more unlike the cre site and progressively more like the HIV site. The final outcome was an enzyme able to excise sequences flanked by the HIV specific pattern.