as you can get a very strong signal with more antibody / less washing / longer incubation
How could you leave out Tyramide Amplification? That alone gives you enough fudge factor to say that something like GAPDH or b-actin are overexpressed in foo vs bar.
But yeah, lets put obvious and well known stuff on the frontpage of/.:-)
Better yet, lets put up obvious and well known stuff that confuses the shit out of people on the frontpage of/.
The idea is that the nanomaterials are delivering RNA that compliments endogenous mRNA - the resulting double stranded RNA is degraded and the protein isn't made. I think TFA mentions that, and if it didn't it should.
This has been my fav for a long time. For a total n00b, random map generator (RMG) capture the flag shold work out pretty well since you don't have to worry about getting lost or getting owned constantly by some fool who's got the map totally memorized.
Tied to the first question: How will the nature of computing, and how we perceive it, change due to biology integration? More to the point, how much of the theory we learn today may change?
IANACS (I'm a biologist) but, Most of today's models of computing still go back to Turing. New models of computing will most likely spring up in the future that are based on the function of biomolecules, metabolic pathways, et al. Work has already been done showing how gene regulation is analagous to boolean expressions.
Linux itself is a clone of an operating system that is 20-plus years old. That's what it is. That is what you can get today, a clone of a 20-year-old system.
So then by your reasons women before suffrage, and minorities before the civil rights movement are just as underprivilaged and unrepresented in Congress as the corporations represented by the RIAA and MPAA are today? Riiiiight.
because they are in store for some seriously intense ass f***ing.
Going after P2P services and other individual "offenders" is probably akin to a bully beating up some kid he doesn't like after school, but what the MPAA and RIAA have in store for themselves if they actually go though with this is more like gang warfare.
Having some 1337 5kr1p+ k1d33 deface the sony music website for having their ability to download free music and movies taken away is nothing compared to someone with real skills seeing that sony "donates" millions to the EFF. If I were the MPAA I'd have 3 monkeys to protect my own assets for each monkey I had trying to DoS my customers.
Or how's this for an incredibly origional idea: don't start stupid sh*** like this and nobody will start any sh*** with the MPAA- then the MPAA won't have to guard their ass like a 98lb prision innmate with "bitch" tattooed on his forehead.
Good equipment based on questionable methodology still produces questionable results.
The idea is that the nanomaterials are delivering RNA that compliments endogenous mRNA - the resulting double stranded RNA is degraded and the protein isn't made. I think TFA mentions that, and if it didn't it should.
No, you just use RNA that compliments mRNA for some required housekeeping function.
This has been my fav for a long time. For a total n00b, random map generator (RMG) capture the flag shold work out pretty well since you don't have to worry about getting lost or getting owned constantly by some fool who's got the map totally memorized.
How old is x86 again?
So then by your reasons women before suffrage, and minorities before the civil rights movement are just as underprivilaged and unrepresented in Congress as the corporations represented by the RIAA and MPAA are today?
Riiiiight.
Going after P2P services and other individual "offenders" is probably akin to a bully beating up some kid he doesn't like after school, but what the MPAA and RIAA have in store for themselves if they actually go though with this is more like gang warfare.
Having some 1337 5kr1p+ k1d33 deface the sony music website for having their ability to download free music and movies taken away is nothing compared to someone with real skills seeing that sony "donates" millions to the EFF. If I were the MPAA I'd have 3 monkeys to protect my own assets for each monkey I had trying to DoS my customers.
Or how's this for an incredibly origional idea: don't start stupid sh*** like this and nobody will start any sh*** with the MPAA- then the MPAA won't have to guard their ass like a 98lb prision innmate with "bitch" tattooed on his forehead.