Transgenic Zebrafish Produced Using Cultured Sperm
understyled writes "According to this article, researchers from Fukui Prefectural University in Obama, Japan, and the National Human Genome Research Institute have produced genetically modified zebrafish using sperm cells grown "in vitro." "The secret to our success was the idea of placing a layer of special 'feeder cells' under the spermatagonia in the laboratory dish. These feeder cells, derived from zebrafish testicular cancer cell lines, promote the growth of spermatagonia and stimulate them to mature into functional sperm," said Dr. Sakai, the study's senior author and a reproductive biologist."
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