DIY Biologists To Open Source Research
destinyland writes "Falling costs and garage tinkering are creating a grass roots movement of amateur biologists whose research is more transparent than that of academia. They are building lab equipment using common household items and even synthesizing new organisms, and their transparency also allows the social pressure which creates more ethical research. DIY Bio.org fosters lab co-ops for large equipment and provokes important discussions. (Would it be ethical to release a homegrown symbiote that cures scurvy in hundreds of thousands of people?) This movement could someday lead to bottom-up remedies for disease, fuel-generating microbes, or even a social-networked disease-tracking epidemiology. 'In much the same way that homebrew computer science built the world we live in today, garage biology can affect the future we make for ourselves,' argues h+ magazine, which featured the article in their summer issue."
I've done this very successfully using liver instead of spit. You get much more dna that way, since you're using chunks of cellular matter instead of just a few stray epithelial cells.
A few words of advice- Use everclear instead of rum. Get it as cold as possible; I used a salt/ice bath. The colder the better. Instead of pouring the alcohol into the glass, decant it using a glass stirring rod or something similar ( http://www.csudh.edu/oliver/demos/gravsulf/pour-r2.jpg ). Do this as slowly as possible. Pre-chill your stir rod to keep the alcohol cold. If you use cellular matter like liver or meat, grind it with a mortar and pestle. A spoon and a bowl will work in a pinch.
It is a very neat experiment to do with kids around, since you can see the results and there's nothing too toxic involved.
-b
No offense, but I've stopped responding to AC's.