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Gene Therapy Approach 'Completely' Protects Mice From HIV Infection
Pierre Bezukhov writes "Scientists from the California Institute of Technology have come up with a gene therapy approach that has proven effective in protecting mice (with humanized immune systems) against HIV infections. They used a genetically altered virus to infect muscles cells and deliver DNA codes of potent antibodies isolated from the blood of human HIV victims (abstract). The muscle cells then began to manufacture the antibodies in quantities that proved 'completely protective' against HIV infection. By contrast, traditional vaccines have not worked against HIV, as scientists have failed to find a molecule that induces the immune system to produce enough potent antibodies. The difficulties stem from the fact that HIV disguises some of its external structures from the antibodies."
I love how you decided to keep this comment anonymous. 'It's as if' you are scared to actually back up your opinion. Seriously, in the 'playground' version of being a fag, you are the biggest one I've seen.
Gravity - 'It's as if nature doesn't think' humans should fly, but we do (planes).
Fun fact - nature isn't CONSCIOUS. It doesn't really give a crap what you think or do. Nature didn't wake up one day and think 'hey, I think I hate queers today!'. Unless you're religious, then of course why I even waste my breath is beyond me.
Zuki: Technical Tomfoolery
1: Allergic reactions tend to develop over time. You may not be allergic at first, but as a result of constant exposure, you may become so over time. Antibodies are small and soluble enough that this isn't usually a problem, but with a non-self fC region, they can be. We've seen this occasionally with the purification tags used in many common biotherapeutics; if your immune system begins to recognize the tag (his-6 is common) from one therapeutic, any other biologic with the same tag will cause an allergic reaction. Note: this doesn't normally occur with antibody-based therapeutics since they can be efficiently purified without being tagged.
2: The thing about turning it off is that the off switch has to be hit in the same cells that are producing it; i.e. millions of separate sites. More gene therapy is unlikely to hit *all* of the same cells, and will potentially cause some unintended consequences in non-target cells. The solution is to use a molecular switch. If I were designing it, I'd flank the antibody promoter region and the transcription start site with loxP recognition sequences. Add a cre recombinase gene under the direction of an antibiotic-activated promoter. Basically this would allow you to reverse at least a portion of the gene therapy at will. Take an antibiotic and the affected cells would actually cut out the antibody producing region they introduced. Unfortunately, if you wanted to again have protection, you'd have to undergo the gene therapy again. You could do a similar setup using siRNA under a controlled promoter, but this scheme also has its drawbacks (repression of the transgene therapeutic would only be temporary - which could be better or worse depending on your goals). Anyway, whichever scheme you use will have to be introduced in conjunction with the original treatment; adding it afterwards won't work nearly as well due to the difficulty in getting the treatment to all of the affected cells.
This is *great* news for gay mice. Ever try to put on one of those tiny little condoms without tearing it with your claws?