Domain: syntheticbiology.org
Stories and comments across the archive that link to syntheticbiology.org.
Comments · 9
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Synthetic Biology (frankenbugs!)
Turns out a lot of the coolest results in synthetic biology have been produced by teams of college students for less than $1500. See the iGEM competition http://2010.igem.org/ (and follow links to older competitions), order some biobricks from New England Biolabs http://www.neb.com/nebecomm/products/productE0546.asp , and check out the tutorials at http://syntheticbiology.org/ It's all open source, too. The price of DNA sequencing and DNA synthesis are both dropping exponentially. It's a lot like the Homebrew Computer Club times in molecular biology right now...
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Linkdump
(AC, so I'm not as much a karma whore)
http://diybio.org/ - open source hardware, biology, XMLizing lab protocols, the goods.
http://openwetware.org/
http://biopunk.org/
http://syntheticbiology.org/
http://partsregistry.org/
http://igem.org/ (international genetically engineered machines competition)
http://ponoko.com/
http://shapeways.com/
http://thingiverse.com/
http://instructables.com/
lifeboat foundation (AKA "fearmongers click here")cat * >
/dev/trend-spotting-machine -
Article is uselessMost informative part:
The researchers explained that the transplantation method is simple in concept, though complicated to execute. First, the proteins were stripped from the M. mycoides LC cells, resulting in naked DNA that can be passed between cells. Then this intact DNA was incubated briefly with M. capricolum cells, soaking in a solution that caused the M. capricolum cells to fuse together. As two of these recipient cells fused, they sometimes encapsulated a donor DNA chromosome.
And then the citation:
Lartigue, Carole, Glass, John I., Alperovich, Nina, Pieper, Rembert, Parmar, Prashanth P., Hutchison III, Clyde A., Smith, Hamilton O., and Venter, J. Craig. Genome Transplantation in Bacteria: Changing One Species to Another. 3 August 2007, Vo. 317, Science.
Abstract:Originally published in Science Express on 28 June 2007
But would it be too painful to actually add in relevant information from the published article? Not all of us know where to go get "Science", nor do we have magical access. Slashdot editors, if you would be so kind- stop accepting articles about papers behind paywalls. Some of us want to actually discuss the contents of these articles, the research methods, to look into what's actually going on
Science 3 August 2007:
Vol. 317. no. 5838, pp. 632 - 638
DOI: 10.1126/science.1144622
Genome Transplantation in Bacteria: Changing One Species to Another
Carole Lartigue, John I. Glass,* Nina Alperovich, Rembert Pieper, Prashanth P. Parmar, Clyde A. Hutchison, III, Hamilton O. Smith, J. Craig Venter
As a step toward propagation of synthetic genomes, we completely replaced the genome of a bacterial cell with one from another species by transplanting a whole genome as naked DNA. Intact genomic DNA from Mycoplasma mycoides large colony (LC), virtually free of protein, was transplanted into Mycoplasma capricolum cells by polyethylene glycol-mediated transformation. Cells selected for tetracycline resistance, carried by the M. mycoides LC chromosome, contain the complete donor genome and are free of detectable recipient genomic sequences. These cells that result from genome transplantation are phenotypically identical to the M. mycoides LC donor strain as judged by several criteria.
The J. Craig Venter Institute, Rockville, MD 20850, USA.
* To whom correspondence should be addressed. E-mail: jglass@jcvi.org ... not this hype that tells us nothing and wastes our time. ("You must be new!")
Anyway, genome transplantation means that maybe we can get the genome of our stem cells transplanted into bacteria. Just store lots of stem cell DNA, and then one day start the procedure to make the bacteria uptake the DNA and--- well, the current problem with this is that the human genome is much different from bacterial genomes, and so there will undoubtedly be way too many problems with the host bacteria, i.e. trying to make some of the proteins and biomolecules that actually causes self-destruction, but the concept/hope is still there.
BTW, the group that this article is about has been taking up way too much of our collective attention:
* Team claims synthetic life feat
* Venter Institute claims patent on synthetic life
* and now this.
And I should probably link over to this site. -
Re:BioBrick?
Thanks for your enlightning hint, but i wanted to know what a "BioBrick" normally is:
http://syntheticbiology.org/BioBricks.html
http://openwetware.org/wiki/BioBricks_construction _tutorial -
Re:BioBrick
"The Registry of Standard Biological Parts is a collection of parts: sequences of DNA with specific function that can be combined together to implement more complex functions. These parts are called BioBricks."
http://syntheticbiology.org/BioBricks.html -
the world of synthetic biologyCheck out the world of synthetic biology
From the FAQ:
Synthetic biology studies how to build artificial biological systems for engineering applications, using many of the same tools and experimental techniques. But the work is fundamentally an engineering application of biological science, rather than an attempt to do more science. The focus is often on ways of taking parts of natural biological systems, characterizing and simplifying them, and using them as a component of a highly unnatural, engineered, biological system.
Neat comic strip by Drew Endy: http://mit.edu/endy/www/scraps/comic/AiSB.vol1.pd
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Re:For those who care
For those who care: CODA Genomics will quite literally will build you a gene (as in here's the test tube full wet stuff, Dr. DNA) if you give them the amino acid sequence, any sequence at all, from an actual organism or something you just made up. Moreover, they guarantee their stuff is the real deal and will generate protein in whatever organism you want.
Synthetic Biology is emerging as a new discipline to study biology in a different way. A sort of 'ground up' building of biological units.
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Re:I am all for this
See previous slashdot discussions of "Open Source Biology And Its Impact on Industry" (slashdot discussion) from 2001, and for how fast costs are falling and skills are spreading "The Pace and Proliferation of Biological Technologies" (slashdot discussion) from 2003.
I am, by the way, posting this from the Synthetic Biology 1.0 Meeting at MIT. Open source is under discussion, as are the risks. -
Synthetic Biology
I would ignore moving from medicine to computers right now, for reasons I'm sure other posters will mention in detail.
The next big technological revolution will be synthetic biology, harnessing the power of organic processes in the same fashion that computers harnessed the power of inorganic logic circuits.
Especially if you already have done classes in organic chemistry, human enzyme pathways, and genetics, you have the base to begin to move into this evolving industry. The first large international conference on synthetic biology will occur in June at MIT.