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Identifying World's Species With Genetic Bar Codes

Fokker writes "Reuters reports that scientists from around the world launched a project on thursday to genetically identify species using bar codes. By taking a snippet of DNA from all the known species on Earth and linking them to photographs, descriptions and scientific information, the researchers plan to build the largest database of its kind."

35 of 156 comments (clear)

  1. what about the zebras? by Suburbanpride · · Score: 5, Funny

    Wont this hurt the self esteem of the animals that already have barcodes?

    --
    sorry 'bout the mess...
    1. Re:what about the zebras? by ikkonoishi · · Score: 3, Funny

      Zebras will be eliminated under the DMCA.

  2. Why? For Check out lines? by DanThe1Man · · Score: 5, Funny

    "Could I get a price check on a large brown marsupial?"

  3. The result will be by hashish · · Score: 5, Interesting

    that we will find more unique animals that we didn't know were unique. We are still identifying more unknown species every day, now with DNA species we thought are related will now be determined not to be.

    1. Re:The result will be by koekepeer · · Score: 2, Informative

      i wonder which specific DNA sequence they use, since it should be present in all species and sufficiently divergent to discriminate between species.

      another thing that might be problematic, is the dicrepancy between different classification methods... sometimes morphological or biochemical classifications do not coincide with DNA based classifications.

      i like the effort, but i have to see it work before i am convinced of the merits. the article is a bit vague regarding implementation.

    2. Re:The result will be by extensis · · Score: 5, Informative
      I have to admit i ahve yet to read the article, but did write a lengthy report on the subject last semester...
      The region of DNA they are using as a barcode is a 561 bp stretch of the cox1 gene, which encodes Cytochrome C Oxidase subunit 1.
      The gene is found in the mitochondrial DNA in all animals and plants, and many homologues are in bacteria and other forms of life.
      There are some great resources out there

      http://barcodinglife.com/, The official site.
      Or for the geeky, http://www.ncbi.nlm.nih.gov/, GenBank, search for cox1, it's the second most sequenced gene on the planet,
      you'll find it's sequence and other info you can play with 'bioinformatically'.

      If anyone is interested Cytochrome C Oxidase is the final protein in your electron transport chain.
      It is the enzyme that reduces oxygen in your body (try breathing in!), and is found in 'nearly' all life that lives in oxygen.

      --
      Mike Jones-{ Genetic Engineer, in Training }-
    3. Re:The result will be by vitamine73 · · Score: 2

      "i wonder which specific DNA sequence they use, since it should be present in all species and sufficiently divergent to discriminate between species."

      True if they actually want to infer a phylogeny of all those species (the so-called Tree of Life). Choosing the adequate data is only one of the problems for those seeking to do this, among other you have huge computational problems (give use faster computers please!) as well as biological problems such as: is the history of life adequatly represented by a tree ? (if the answer is no, the computanional problems get worse since networks are much more complex than trees).

      Also, incongruence can arise not only because different data sets are used, but also because different optimality criteria are used in phylogenetic reconstruction. Evolutionnary biologists have used and still use today a plethora of different such criteria as implemented in the many distance methods (clustering and least-squares), maximum parsimony methods, maximum likelihood methods and now bayesian methods (check out Joe Felsenstein's page to see some of the software available to phylogeneticists).

      However the authors seems to only want to identify species. From their web site : "DNA-based systems promise to revolutionize the task of identification by providing reliable, inexpensive, and rapid diagnoses of species identiy".

      Finally, remember that classification (puting organisms in russian dolls that are called species, genus, family, order, class and phylum) and phylogeny (infering the phyletic relationship between groups of living organisms, usually in the form of a branching diagram - i.e. what you refer to in your comment) are not the same thing. Classification tend to be based on phylogeny, but they need no be.

  4. Um... GenBank? by davidstrauss · · Score: 5, Informative
    That project, minus the insignificant addition of photos, is already widely underway at GenBank, a part of NCBI, which is in turn part of NIH. Most major biological journals require submissions of sequences to GenBank prior to consideration for publication. Combined with NCBI's taxonomic system, I don't see the value of this new project.

    I work in this lab. Specifically, I'm working on objective analysis of RNA sequence alignment heuristics.

    1. Re:Um... GenBank? by dan+dan+the+dna+man · · Score: 2, Insightful

      NCBI's taxonomy is widely regarded to be.. well suspect by people working in the taxonomy field. In fact there is NO common taxonomy system, contrary to popular belief. Like many other areas of science it is beset with individuals who *talk* co-operation and fail to do it.

      I have seen taxonmy comparisons from various sources and NCBI's is by no means definitive.

      --
      I don't read your sig, why do you read mine?
  5. I have never heard of a technology more.. by ciroknight · · Score: 2, Interesting

    ..based on something from Star Trek. This is the very kind of thing I think of when they pull up their tricorder to some alien race, and poof, a strand of their DNA is up on one of those pretty LCD monitors behind them.

    That being said, I think this is a brilliant use of computer technology. Catalogs and databases of this kind are what we need, especially while going through the jungles of various continents in search of miraculous wonder cures. Besides, if we had done this before with the Dodo and other animals that went extinct, we could rebuild the strands of DNA, and make the animal again for study. I tend to think more and more as we corrupt the planet with roads and sidewalks that research and food will be the only reason to have living stuff around, and knowing that we have a computer database of all that is alive and the ability through cloning to reproduce any of it is at least a comforting thought.

    end rant.

    --
    "Victory means exit strategy, and it's important for the President to explain to us what the exit strategy is." G.W.Bush
    1. Re:I have never heard of a technology more.. by karakal · · Score: 3, Interesting

      No, this is exactly NOT what we need. This is something a geek needs. You can't compare species with their DNAs. This is a great misbelief. An animal is more than just a little DNA (the same as a human). But this is one further example of throwing some technology and money at a subject, which it doesn't need this and destroying it therefore.

    2. Re: I have never heard of a technology more.. by Black+Parrot · · Score: 2, Funny


      > ..based on something from Star Trek. This is the very kind of thing I think of when they pull up their tricorder to some alien race, and poof, a strand of their DNA is up on one of those pretty LCD monitors behind them.

      But that's a few hundred years in the future. Right now the Federation still uses the older Anal Probe (tm) technology, to the great mortification of species all around the galaxy.

      --
      Sheesh, evil *and* a jerk. -- Jade
    3. Re: I have never heard of a technology more.. by Ohreally_factor · · Score: 2, Funny

      Right now the Federation still uses the older Anal Probe (tm) technology, to the great mortification of species all around the galaxy.

      I hate to break it to you, but that wasn't really Dr, McCoy that examined you.

      Still, I bet you're really popular at the cons.

      --
      It's not offtopic, dumbass. It's orthogonal.
    4. Re:I have never heard of a technology more.. by Chreo · · Score: 2, Informative
      You can't compare species with their DNAs. This is a great misbelief. An animal is more than just a little DNA (the same as a human).
      I don't exactly know what you put in the word "compare" here but using the common understanding then that is what we do for research. http://phylo.gen.lu.se/

      You can perfectly well compare the sequence of two homologous genes from two species and knowing the function of one you can guestimate the function and domains of the other.
      --

      Life is what happened when Good Intentions met Harsh Reality (the brother of the more infamous Chaos).
  6. The hard part is... by Lynx0 · · Score: 4, Informative

    ...actually finding and agreeing on the locus (piece of DNA) to use for this. It has to be present in all species, i.e. in gnat and cow and jellyfish, but also has to be variable enough to be able to differenciate between, for example, the Tennesse gnat and Alabama gnat.
    The main problem is that the locus must contain two regions to the right and to the left of the sequence of interest that must be highly invariable. This is necessary so that the same PCR primers can be used to amplify the sequence in most species. The amplification step is necessary for sequencing.

  7. how long until by mirko · · Score: 2, Insightful

    sombody thinks about determining human species based on their DNA ?

    --
    Trolling using another account since 2005.
    1. Re:how long until by espressojim · · Score: 3, Interesting

      Well, you may want to look up Admixture mapping to start. We don't really have species, but we have people from various backrounds with differing genetics that cluster by location due to population movements, bottlenecks, and migration.

      Or: Yeah, you can tell the difference between say...chinese and japanese people by looking at the frequency of various single nucleotide polymorphisms.

  8. Pet store efficiency by Orgadam · · Score: 5, Funny

    Does this mean the pet stores of the future will allow a cashier to swipe an animal across an electric eye, greatly increasing the efficiency and speed with which people can buy large quantities of animals? Such a system could greatly help out my habit of buying as many mice as I can! Although, it becomes a problem when geneticists demand that barcodes be placed on individual fruit flies so that they, too, can be bought quickly and efficiently.

    Yeah, I know the barcodes won't be put on the animals... but maybe after a trial run, customers will demand it!

  9. A Few Less Species by _Hellfire_ · · Score: 3, Insightful

    The sad part is that by the time they get around to barcoding all of them, we'll have a few less species around to bar code.

    --
    "And then I visited Wikipedia ...and the next 8 hours are a blur..."
  10. Open Directory by wces423 · · Score: 5, Interesting

    Wikipeople have free online directory of species WikiSpecies

  11. lets say they actually do it... by Anonymous Coward · · Score: 2, Interesting

    ... the find ever species and make a bar code for it. Then what ? Call me dumb - but the point of a barcode is to easily scan it .. last time i checked, frogs dont have barcodes on them.

    You find a weird insect .. 'oh - i'll just check the barcode database! oh wait, it doesnt have one .. damn'

    scrap the barcode thing .. just build the database (perhaps spending more time (and cash) on it you could have the full DNA sequence .. not just a scrap)

    So next time we wipe out some endangered swamp monster we have a backup ;)

    1. Re:lets say they actually do it... by Dragon+Rojo · · Score: 2, Funny
      You find a weird insect .. 'oh - i'll just check the barcode database! oh wait, it doesnt have one .. damn'

      That's what the Pokedex is for

  12. Re:Why? For Check out lines? by mattjb0010 · · Score: 3, Interesting

    There's actually a real lack of genetic data on marsupials and monotremes. Interesting for evolutionary reasons, but also because understanding how koalas break down toxins (such as various eucalyptus oils) gives general insights into drug metabolism (there are people studying this in my lab, in fact). If you'd like to donate funds for sequencing the kangaroo, go here.

  13. Re:Why? For Check out lines? by commodoresloat · · Score: 2, Funny

    Best thing is they will scan the animal using a :cue:cat.

  14. Partially useful by onco_p53 · · Score: 5, Informative

    The fragment of DNA that they are sequencing is located on the mitochondria and is part of the cytochrome c oxidase gene (COI). http://en.wikipedia.org/wiki/DNA_barcode

    I am a taxonomist and can tell you this is of limited usefulness, sure we will be able to see some differences between organisms, but this is already done is many studies with the control region of the mitochondria.

    The problem could be if people just rely on these sequences alone to delineate species, we learnt this years ago in bacterial taxonomy that you simply cannot rely on any one particular gene (There is evidence of horizontal gene transfer in 16S genes if someone was going to counter with that).

    A speciation event is driven by some environmental pressure (change in temperature say). The genes that are under selection of this pressure will change at different rates to one under no direct pressure (COI).

    I could rant on but, I think this project is of some use only if combined with traditional taxonomy. The danger is that a large flashy project like this will steal research dollars away from traditional taxonomy.

    1. Re:Partially useful by Lars+Arvestad · · Score: 2, Interesting
      That is a good wikipedia link you gave. I recommend it to people interesting in this thread.

      I could rant on but, I think this project is of some use only if combined with traditional taxonomy. The danger is that a large flashy project like this will steal research dollars away from traditional taxonomy.

      It is not like the field of taxonomy is without flashy projects. Consider the Tree of life project for instance.

      Given the information in the Wiki you referred to, wouldn't you say that the barcodes could provide a starting point for good taxonomy projects?

      --
      Reality or nothing.
    2. Re:Partially useful by Lynx0 · · Score: 2, Interesting

      The barcode sequence will only be a means of identifying the species, not to find new ones or do taxonomy on them. The first connection between the sequence and the actual animal will be done by traditional taxonomy, because there is of course currently no other way do differenciate between many species than by the help of a specialist for the group.
      But once this is done, if I find the beetle (or something) again, I can identify the species by myself with a PCR and sequencing, and don't have to mail it to a specialist who counts some bristles under the microscope for hours.
      It's about exchanging the really hard do interpret morphology with sequence data as a means of identification.

  15. one use for this technology by hengist · · Score: 5, Interesting

    Where I work, a group is using this technique to identify insect pest species.

    If, for example, a group of insect eggs, or even just some parts of an insect like the legs, were found in a shipping container, then it can be extremely difficult to identify the insect species by morphology. By sampling and barcoding the DNA in the eggs or fragments then it can be determined which insect was present, and therefore whether a potential pest was present in the container.

    For a country that depends on agriculture, like New Zealand does, this is a very important technology.

  16. Taxonomic debates? by Claire-plus-plus · · Score: 2, Interesting

    Maybe projects such as this one might solve some taxonomic debates such as the old chestnut about whether Megachiroptera (fruit bats) and Microchiroptera (insectivorous bats) are acutally closely related.

    For those of you who don't know the debate is as to whether Megachiroptera are closer related to primates than they are to the Microchiroptera. I personally believe that fruit bats are flying monkeys. Therefore, fruitbats are the only primates native to Australia (besides Homo Sapiens obviously).

    --
    99 bottles of beer in 175 characte
  17. Re:Just Wondering... by ikkonoishi · · Score: 2, Interesting

    Maybe one like this

  18. X5? by Claire-plus-plus · · Score: 2, Funny

    Well one species already carries it's own barcode. It's always there on the back of their necks and grows back when it's removed. And one of them (X5-452) is a hottie as well.

    --
    99 bottles of beer in 175 characte
  19. Barcoding web site by lovebyte · · Score: 4, Interesting

    barcoding.si.edu
    From their DNA barcode page:
    DNA barcode sequences are very short relative to the entire genome and they can be obtained reasonably quickly and cheaply. The cytochrome c oxidase subunit 1 mitochondrial region (COI) is emerging as the standard barcode region for higher animals. It is 648 nucleotide base pairs long in most groups...

    Let us note however that most of our planet's biodiversity is contained in the 60% of the biomass that people don't talk much about: Bacteria. Most of them live in the soil and are difficult to study and are simply unknown. It would seem that this barcoding project does not include Prokaryota ( = Bacteria,Archaea) unfortunately.

    --

    I'll do it for cheesy poofs.

  20. What just bit me? by wolf- · · Score: 3, Funny

    Martha, I've been bitten by a snake!

    Hang on Frank, let me get my barcode reader out..

    *eh* *eh*
    Dang it, frank, hold its head still. Can't get a read.

    *bleep* *bleep*
    There we go.

    Good news Frank, its only a black mamba

    Martha, how is that "good"? I thought they were deadly.

    Frank, the life insurance is all paid up!

    --
    ----- LoboSoft specializes in Digital Language Lab
  21. Re:Obligatory by gregsweb · · Score: 2, Funny

    Don't you have to get them good and drunk before trying??
    --------
    I'm not fat.. I'm just big boned!

    --
    I'm not fat.. I'm big boned!!
  22. Get out your foil hats! by bradleyland · · Score: 2, Funny

    The barcodes are coming! The barcodes are coming!