For proteins, stability ranges across the whole spectrum. Some nanostructures fall apart if the salt concentration is just a little off, while others will be just fine near boiling: there are viruses that survive great in the geothermal features in Yellowstone.
You've got to admire TAQ, even if it never amplifies what I want:-) But that illustrates my point, proteins are of course versatile in structures whereas DNA is not. It doesn't seem like there's any protein component to these cubes, and nothing I know of in DNA can crosslink strands like cystein bridges (besides holliday junctions, and they don't seem to be using them here.) It seems like you could design a strand of DNA that theoretically would form a box based on kinks in the helix, but I don't see how it would be held together in real solutions.
I'm definitely going to have to read the real article when I have access.
In contrast, DNA can be made more or less fully synthetically, and the misfolding problem is a non-issue: it can be melted down and re-folded nearly infinitely.
See I was thinking the opposite, that the misfolding problem would be much bigger (though I'm not a biochemist and you are.) What's making sure the DNA folds back into the highly ordered secondary structure you're aiming for? DNA denatures and renatures mainly in the pairing, the secondary structures seem like they're much weaker and more promiscuous than protein structure. I would expect the box to fall apart much easier than a protein box, but again, that's not an expert opinion.
Does the immune system actually react to extracellular DNA? I've never heard of that happening. Wouldn't be that much of a threat, DNA by itself doesn't do much besides sit there and likely get chewed up by nucleases. But IANAIB (where IB stands for immunobiologist or whatever they call themselves).
... so are you actually spreading FUD about technology which has no actual applications yet?
Site is slashdotted. Or just offline due to bankrupcy.
Re:You stole my joke; oh well, it's "Did not finis
on
Duke Nukem For Never
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· Score: 1
Do not fund... a game that has been in development for over a decade.
Seriously, I can't wait for the book/interview explaining how this lasted so long. How did it keep getting strung along? Zero accountability over at 3d realms? Unwillingness to the point of insanity, to admit you've wasted a lot of money and time and should be fired?
At a minimum, I'd expect the programmers and graphic designers to quit. Doesn't "Worked 10 years on a game that was clearly never going to be released" look kind of bad on your resume?
Where that is defined as deserts which are advancing, and whose advance is not containable by substitution of sustainable farming practices for unsustainable ones...
Kind of a trivial semantic argument right there. Whatever the cause, whatever you call it, it's not good for people who are going to be living in sand soon.
There is no such evidence. All that is needed is sensible traditional mixed farming. And a lot less journalistic blather about desertification that is not happening, global warming that is not happening, and how the one imaginary event is a consequence of the other imaginary event. And for well meaning idiots to stop subsidizing goats.
It would be nice if they practiced responsible farming, yes. Why isn't that happening already? Is there another problem upstream of unsustainable farming practices that's causing everyone to farm stupidly? Like maybe dumb economic systems that make it such that anyone who farms anything besides goats is quickly going to lose the farm and be replaced by someone who only raises goats?
I don't know if that's the case or not, but I do know that simple answers, like the one you just gave, never work on complex problems, like the one being discussed.
Please, stay in the coffee shops in the village, discussing the importance of your latest pathetic attempt at relevance through putting mannequin arms in toilets bowls and calling it art.
Problem is, they have the internets in those coffee shops. So they stay in their version of starbucks but with their macbooks and iphones, their attempts at proving that a little bit of knowledge can be the most ridiculous thing spread.
I propose we build a wall out of bacteria and myspace pages to stop the "artistification" of the internet.
To clarify THAT, one hypothesis is that there are stem cells in tumors. This makes it tough to treat tumors, as the cells you really need to get are the stem cells seeding the tumor, but they tend to be missed by a lot of chemotherapy drugs as they may be slower-dividing, as stem cells may be in other contexts.
There are some cancers that may arise from normal populations of stem cells as well, but no one is saying all cancerous cells came from a population of stem cells. No one is saying all cancers have stem cells keeping them going either.
Note that's all theory, some of it may be outdated, some or all of it may have been disproven. I'm not too up to date, and stem cell biology moves really fast compared to most other fields in bio.
So would that mean if you wanted to commit a crime, you would want to admit to it since if the 5th amendment applied they would not be able to use your blog as evidence against you?
To put the joke aside for a moment, I thought the 5th gave you a right not to incriminate yourself, it didn't mean that if you did incriminate yourself (like on a blog) it couldn't be used against you.
Going with the joke, I did say it would be a challenge. Somone more creative than me should get on that.
It's a good start, but lets try to get ALL the amendments!
Next up: the third amendment. You know, because calling a blog a weapon isn't much more logical than saying being forced not to blog is the same as being forced to quarter the armed forces. The fourth amendment, search and seizure shouldn't be as much of a leap. Getting it to qualify under the 5th is going to be the first real challenge.
I think we should stop at the 12th though. Don't really see how dates of presidential elections can be applied to blogs.
I've always thought we could come up with a better term than "fanboy." What's wrong with being a fan of something? And most people who play games are guys. Anyway, I guess we're stuck with it now.
If you think Sculley's Apple will make a comeback then you're mistaken and don't know history.
Overstatement alert: not knowing what it's like in the board room of apple does not make you ignorant of history, it makes you ignorant of apple current events. While that isn't as bad as not knowing history, it is still a little bad, because those who don't know the state of leadership at apple computers are doomed to not really give a shit.
Er... that's a bit of a reach. Not that it's not a ripoff of nintendo, but really does THAT affect the quality of the console?
To use a car analogy: "This is a terrible car... muffler missing, no battery, two broken headlights, there is a barbie doll in the glove box, and the front axle is broken."
On second thought, I did miss this
Stupid and gigantic external power brick
So maybe that car metaphor should include "has one of those stupid dreamcatchers on the rearview mirror."
I thought the same thing, plus the following: who cares about either as an end user. "Waaa! These controllers are too similar, I'm always trying to plug the Xbox controller into the PS2 and vice versa!"
What's so bad about similar controller designs? Do you hear people complaining about how the keyboard and mouse for a PC is so similar to the keyboard and mouse for the mac? No, it makes sense that they're going to be similar, convergent evolution, good design is good design.
Too many games made for both systems? Put that another way: there were too few system exclusives for GP. Who the hell LIKES system exclusives besides the console companies themselves?!? "Woo! I don't get to play the game I want on the console I own! Awesome! Consumer choice sucks, hooray for monopolies!"
I think someone has pride in one console or the other. Which is strange, because they're things you buy, not something that should affect your identity. Then again, I don't understand people who have pride in their local sports team, and a lot of people do, so maybe I'm off here...
which companies does Obama think could afford to pay those taxes?
The ones that AREN'T going broke. You mentioned two that are. Those the only two american corporations you know of?
Raise $200 billion more in taxes and pay $200 billion more in bailouts, is that how it's supposed to work?
While I think that's an oversimplification, closing them now but bailing them out would mean that when bailouts are no longer needed, we'd still have the loopholes closed.
No, thank them. Obama, being a fool, is about to learn why those loopholes exist. We put them in to keep some of the multinationals headquartered here in the US in practice by allowing them to headquarter on paper somewhere else and we all agreed to ignore the oddities that followed from that. Forced to actually choose many will opt to close up the skyscraper here and open one up in a more business friendly climate. Then profits from US operations will flow OUT instead of overseas profits flowing IN. I'm failing to see how the US wins.
You know, I think if we allowed them loopholes to ALL laws, that might encourage more investment.
2. Whiskey must be stored in oak barrels to age. Once it is out of the barrel, and in a bottle or steel vat, it no longer ages. So a 10 year old whiskey sitting in a bottle for 50 years is still a 10 year old whiskey.
Are there any other laws of physics that whiskey violates? No wonder there are so many scottish physicists.
Slashdot Mirror
For proteins, stability ranges across the whole spectrum. Some nanostructures fall apart if the salt concentration is just a little off, while others will be just fine near boiling: there are viruses that survive great in the geothermal features in Yellowstone.
You've got to admire TAQ, even if it never amplifies what I want :-) But that illustrates my point, proteins are of course versatile in structures whereas DNA is not. It doesn't seem like there's any protein component to these cubes, and nothing I know of in DNA can crosslink strands like cystein bridges (besides holliday junctions, and they don't seem to be using them here.) It seems like you could design a strand of DNA that theoretically would form a box based on kinks in the helix, but I don't see how it would be held together in real solutions.
I'm definitely going to have to read the real article when I have access.
In contrast, DNA can be made more or less fully synthetically, and the misfolding problem is a non-issue: it can be melted down and re-folded nearly infinitely.
See I was thinking the opposite, that the misfolding problem would be much bigger (though I'm not a biochemist and you are.) What's making sure the DNA folds back into the highly ordered secondary structure you're aiming for? DNA denatures and renatures mainly in the pairing, the secondary structures seem like they're much weaker and more promiscuous than protein structure. I would expect the box to fall apart much easier than a protein box, but again, that's not an expert opinion.
Does the immune system actually react to extracellular DNA? I've never heard of that happening. Wouldn't be that much of a threat, DNA by itself doesn't do much besides sit there and likely get chewed up by nucleases. But IANAIB (where IB stands for immunobiologist or whatever they call themselves).
Here's what I'm curious about: how did he get charged with theft and receiving stolen property?
He stole it, then sold it to himself.
He was also charged with sexual assualt, of himself, because when they picked him up he was looking at porn.
I thought more and more convictions were based on ISP logs instead of hard drive searches these days...
I'd bet the RIAA wants to be as invasive and punitive as possible. I'm suprised they haven't asked for daily body cavity searches of all defendants.
Costarring everyone in the RIAA. I'm getting the torrent right now.
Site is slashdotted. Or just offline due to bankrupcy.
Do not fund... a game that has been in development for over a decade.
Seriously, I can't wait for the book/interview explaining how this lasted so long. How did it keep getting strung along? Zero accountability over at 3d realms? Unwillingness to the point of insanity, to admit you've wasted a lot of money and time and should be fired?
At a minimum, I'd expect the programmers and graphic designers to quit. Doesn't "Worked 10 years on a game that was clearly never going to be released" look kind of bad on your resume?
Where that is defined as deserts which are advancing, and whose advance is not containable by substitution of sustainable farming practices for unsustainable ones...
Kind of a trivial semantic argument right there. Whatever the cause, whatever you call it, it's not good for people who are going to be living in sand soon.
There is no such evidence. All that is needed is sensible traditional mixed farming. And a lot less journalistic blather about desertification that is not happening, global warming that is not happening, and how the one imaginary event is a consequence of the other imaginary event. And for well meaning idiots to stop subsidizing goats.
It would be nice if they practiced responsible farming, yes. Why isn't that happening already? Is there another problem upstream of unsustainable farming practices that's causing everyone to farm stupidly? Like maybe dumb economic systems that make it such that anyone who farms anything besides goats is quickly going to lose the farm and be replaced by someone who only raises goats?
I don't know if that's the case or not, but I do know that simple answers, like the one you just gave, never work on complex problems, like the one being discussed.
That was a little too basic. In fact, it's SO basic, I'm not sure that qualifies as a question. I'll attempt to answer it anyway:
Algae!
Please, stay in the coffee shops in the village, discussing the importance of your latest pathetic attempt at relevance through putting mannequin arms in toilets bowls and calling it art.
Problem is, they have the internets in those coffee shops. So they stay in their version of starbucks but with their macbooks and iphones, their attempts at proving that a little bit of knowledge can be the most ridiculous thing spread.
I propose we build a wall out of bacteria and myspace pages to stop the "artistification" of the internet.
why exactly are we to interfer with this process?
Gotta do SOMETHING with all that bacteria and sand.
Put another way: you can say you're a journalist, but that doesn't mean you ARE a journalist.
Mutations mean cancer at worst, not the next fictional zombie threat.
Er... I think zombies actually are worse than cancer. Less likely yes, but still worse.
To clarify THAT, one hypothesis is that there are stem cells in tumors. This makes it tough to treat tumors, as the cells you really need to get are the stem cells seeding the tumor, but they tend to be missed by a lot of chemotherapy drugs as they may be slower-dividing, as stem cells may be in other contexts.
There are some cancers that may arise from normal populations of stem cells as well, but no one is saying all cancerous cells came from a population of stem cells. No one is saying all cancers have stem cells keeping them going either.
Note that's all theory, some of it may be outdated, some or all of it may have been disproven. I'm not too up to date, and stem cell biology moves really fast compared to most other fields in bio.
Missing a "Where's the beef" joke here.
So would that mean if you wanted to commit a crime, you would want to admit to it since if the 5th amendment applied they would not be able to use your blog as evidence against you?
To put the joke aside for a moment, I thought the 5th gave you a right not to incriminate yourself, it didn't mean that if you did incriminate yourself (like on a blog) it couldn't be used against you.
Going with the joke, I did say it would be a challenge. Somone more creative than me should get on that.
It's a good start, but lets try to get ALL the amendments!
Next up: the third amendment. You know, because calling a blog a weapon isn't much more logical than saying being forced not to blog is the same as being forced to quarter the armed forces. The fourth amendment, search and seizure shouldn't be as much of a leap. Getting it to qualify under the 5th is going to be the first real challenge.
I think we should stop at the 12th though. Don't really see how dates of presidential elections can be applied to blogs.
I've always thought we could come up with a better term than "fanboy." What's wrong with being a fan of something? And most people who play games are guys. Anyway, I guess we're stuck with it now.
If you think Sculley's Apple will make a comeback then you're mistaken and don't know history.
Overstatement alert: not knowing what it's like in the board room of apple does not make you ignorant of history, it makes you ignorant of apple current events. While that isn't as bad as not knowing history, it is still a little bad, because those who don't know the state of leadership at apple computers are doomed to not really give a shit.
> A stupid Nintendo Mii ripoff
Er... that's a bit of a reach. Not that it's not a ripoff of nintendo, but really does THAT affect the quality of the console?
To use a car analogy: "This is a terrible car... muffler missing, no battery, two broken headlights, there is a barbie doll in the glove box, and the front axle is broken."
On second thought, I did miss this
Stupid and gigantic external power brick
So maybe that car metaphor should include "has one of those stupid dreamcatchers on the rearview mirror."
I thought the same thing, plus the following: who cares about either as an end user. "Waaa! These controllers are too similar, I'm always trying to plug the Xbox controller into the PS2 and vice versa!"
What's so bad about similar controller designs? Do you hear people complaining about how the keyboard and mouse for a PC is so similar to the keyboard and mouse for the mac? No, it makes sense that they're going to be similar, convergent evolution, good design is good design.
Too many games made for both systems? Put that another way: there were too few system exclusives for GP. Who the hell LIKES system exclusives besides the console companies themselves?!? "Woo! I don't get to play the game I want on the console I own! Awesome! Consumer choice sucks, hooray for monopolies!"
I think someone has pride in one console or the other. Which is strange, because they're things you buy, not something that should affect your identity. Then again, I don't understand people who have pride in their local sports team, and a lot of people do, so maybe I'm off here...
which companies does Obama think could afford to pay those taxes?
The ones that AREN'T going broke. You mentioned two that are. Those the only two american corporations you know of?
Raise $200 billion more in taxes and pay $200 billion more in bailouts, is that how it's supposed to work?
While I think that's an oversimplification, closing them now but bailing them out would mean that when bailouts are no longer needed, we'd still have the loopholes closed.
No, thank them. Obama, being a fool, is about to learn why those loopholes exist. We put them in to keep some of the multinationals headquartered here in the US in practice by allowing them to headquarter on paper somewhere else and we all agreed to ignore the oddities that followed from that. Forced to actually choose many will opt to close up the skyscraper here and open one up in a more business friendly climate. Then profits from US operations will flow OUT instead of overseas profits flowing IN. I'm failing to see how the US wins.
You know, I think if we allowed them loopholes to ALL laws, that might encourage more investment.
2. Whiskey must be stored in oak barrels to age. Once it is out of the barrel, and in a bottle or steel vat, it no longer ages. So a 10 year old whiskey sitting in a bottle for 50 years is still a 10 year old whiskey.
Are there any other laws of physics that whiskey violates? No wonder there are so many scottish physicists.