Agilent (an HP spinoff) has been printing DNA microarrays using inkjet technology on a commercial scale for a decade or so. Arrayjet will sell you an inkjet-based arrayer that can print 'proteins, cell lysates, carbohydrates, lectins, DNA & oligos': http://www.arrayjet.co.uk/products This is not only practical, it's big business.
Not a virologist, but your response doesn't sound kosher. I don't see anywhere in your references, or any scientific citation linked by anyone at this site, anything at all to suggest that Gene VI insertion was at all Intentional.
The point is that the 35S promoter and gene VI overlap in the viral genome, or to put it another way the same bit of sequence has multiple functions (this is common in viruses, which tend to make very efficient use of their genetic material).The sequence in question is at about 10 o'clock in this circular genome map:
The fine inner broken ring is the 35S transcript, and the promoter is at its 'blunt' end. This aligns with the 'sharp' end of gene VI ('TAV', in red).
If you use the promoter in a GM plant (as is commonly done) then you're inevitably also bringing along a fragment of the gene, since it's actually the same sequence. This is not the same thing as inserting the entire gene, of course, though the fragment may have some subset of the gene's multiple known functions. The DNA construct used to make the GM plants is not designed to express this fragment, but there's a possibility than once inserted in the host genome (depending on the surrounding sequence elements), the fragment could be expressed from some other promoter, making a protein that is eventually eaten by us.
An intermission would give me just enough time to think seriously about the horrible decision I've made and how hours of my life would be better spent by going home for a beer and a book.
May I suggest The Hobbit? At 1.4Mb (including the illustrations) the.epub would fit on a floppy and, I suspect, still end up saying more than Jackson's multi-petabyte trilogygasm.
"Why, I feel all thin, sort of stretched, if you know what I mean: like butter that has been scraped over too much bread." - Bilbo Baggins.
If there had been some EASY and OBVIOUS way to get rid of the METRO interface and go back to a Classic Shell she might have been happy with it, but after an hour of trying to do anything useful she wanted it boxed up and she has already returned it for a refund.
It's a useful project that has been adding back features removed by MS from Vista onwards. Their start menu actually pre-dates Win 8 - it's more configurable than the standard Win 7 menu.
Of course, it's crazy that downloading something like this is even necessary!
Astonishing isn't it? They've taken an excellent product (by MS standards) and done their best to bury it under a silly hybrid UI setup. Take 10 minutes to install Classic Shell, configure it to boot straight to the desktop (start menu enabled, hot corners disabled), re-register the file types that have been hijacked by Metro apps, and you have arguably the best conventional version of Windows to date - fast booting, integrated antivirus, upgraded task manager, ISO mounting, and a nice clean theme, etc.
Basically all the bad press could have been avoided if they'd made Metro and the start menu globally optional without third party solutions. IT departments (even if they get past the reviews) will take one look at the default configuration and its unpredictable switches between desktop and Metro, think support calls, and file the whole thing as 'Do Not Want'. That MS are already making noises about Windows Blue for 2013 suggests they've realised there's little chance of widespread corporate adoption for Windows 8.
Lastly, you dont need the start button if you learn how to use whats there.. Thats like complaining when going from Win 3.1 to 95. They got rid of my Program Manager I wish they gave me a way to turn it back on..
They did, from 95 up until XP SP1 ( http://support.microsoft.com/kb/142255 ). Of course back then they actually had people who thought about the impact of new interface design on users:
At one point in the design of Windows 95 they considered having two separate UIs, the windowed interface we know and a separate, simplified interface they thought might be suitable for beginners, and which seems to have featured a set of tiles that launched the various applications. Although the design "tested well, because it successfully constrained user actions to a very small set", it was abandoned because "If just one function a user needed was not supported in the beginner shell, s/he would have to abandon it (at least temporarily)", learning "would not necessarily transfer well to the standard shell", and "users had to learn two ways of interacting with the computer, which was confusing". I wonder if the Windows 8 design team were aware of this document..?
Maybe it's the airlines who are worried about the potential for violence inherent in being stuck next to someone jabbering away on their phone for an 8 hour flight? I hope they block Skype...
Though all my Nikon lenses are AF, I've always been tempted to get the classic 105mm f/2.5 AI (hey, maybe that'll make me shoot like Steve McCurry!). But an 85mm would actually make more sense on my D300 with the DX crop...
I have some of this gear too, including a Leica IIIa that's contemporary with the lens. As you probably know, forward compatibility isn't as good for the bodies (you can't just use an adapter and retain infinity focus with a bayonet lens), but there are a few modern screwmount lenses like those made by Cosina/Voigtlaender, so it's still possible to use pieces of kit made 70 years apart.
That's true, and though there were a few other (now obscure) still cameras that shot 24x36, 35mm as we know it effectively dates from the first production Leicas in the 1920s (which is still a pretty decent run for a tech standard). 'Half frame' (like the movie frame) still cameras were still made as late as the 80s, though, and some (like the original Olympus Pen) were quite popular.
I have a lens made for a 1930s Leica which, using an adapter they started making in the 50s (when the current bayonet mount was introduced), will work happily with any of their later rangefinder cameras, including the latest 2012 digital model (if I could afford it). As a bit of a long shot, I emailed the company a few years ago with a technical query about this lens, and got a prompt response with a request for the serial number so they could check their records! The standard flash/accessory shoe used today is also the same size as the one Leica was using as early as the 1920s, as is the 35mm cassette (so you can stick modern film in that antique Leica).
35mm itself (packaged differently) is basically a 19th century movie film standard, and we're also in the third century of several other common tech standards - the D cell battery goes back to 1898, the 1/4 inch audio jack is a 19th century phone switchboard plug, and the Edison screw lightbulb dates from the same era. Any others?
Most likely it's way too technical for today's/. average reader and editor.
...and probably because the conclusions of the paper have very little in common with the massively hyped version on medicaldaily.com. The original authors are much more cautious (and certainly don't claim that this is _the_ difference):
"Taken together, the unusual features of miR-941 evolution, as well as its potential association with functions linked to human longevity and cognition, suggest roles of miR-941 in the evolution of human-specific phenotypes."
A quick question; why can't I have both mate and cinnamon installed on the same system.
You probably can. A major reason for renaming everything in MATE was to allow co-existence with Gnome 3-based systems (which would include Cinnamon). You'll get one from the installation media, and can install the other from the repositories, then choose which you want to run at login time. However, each is a complete desktop (MATE rather more so) so you probably only need one.
The problem is most any terrestrial network protocol expects a minimal signal-response delay between nodes
RFC 1149 does not assume this, though the current implementation would have to be modified to avoid complete packet loss in a non-terrestrial environment - BF Skinner's work suggests one obvious adaptation:
i just tried it for the first time last night as a possible alternative to unity for new linux users but found problems and then saw why.
I've been using it for about a year, and don't see any major problems. It's my standard desktop on Ubuntu, and on the Mint VMs I have on Windows machines. It works about as well as any other desktop, including the old Gnome 2 - certainly well enough to be adopted by Mint and Fedora. It's faster and more complete than Cinnamon, which is a decent attempt at 'a gnome2 style interface with modern components'.
It should die just because you aren't interested? Mate does use gtk2 right now (just like Xfce, etc.) which is stable rather than unsupported, and continues to receive small updates and bug fixes. As for the other libraries, here's a comment from one of the developers:
"MATE team is working hard to do this and we are in a good point. Migration to gsettings/dbus (and deletion of obsolete libs like bonobo, gconf, gnome-vfs, libgnome, libgnomeui and libgnomecanvas) is almost complete, and GNOME developers helped us on this too."
"We found on St. Paul's only two kinds of birds - the booby and the noddy. The former is a species of gannet, and the latter a tern. Both are of a tame and stupid disposition, and are so unaccustomed to visitors, that I could have killed any number of them with my geological hammer."
Slashdot Mirror
I was the proud owner of a 3rd party (Memotech) low profile RAM pack, bought at WH Smith. No Blu-Tack required!:
http://www.flickr.com/photos/deadkenny/4002315512/
http://archive.computerhistory.org/resources/access/physical-object/2005/04/102642093.01.01.lg.jpg
http://www.zx81stuff.org.uk/zx81/showmag.php?mag=SinclairUser/Issue002/Pages/SinclairUser00200009.jpg
An FPS without any S (or colour, or sound, or high resolution graphics):
http://en.wikipedia.org/wiki/3D_Monster_Maze
http://www.youtube.com/watch?v=nKvd0zPfBE4
Armed with the awesome power of a Sinclair ZX81 and its 16k external RAM pack, you could run around a maze, chased by a dinosaur. In 3D!
Agilent (an HP spinoff) has been printing DNA microarrays using inkjet technology on a commercial scale for a decade or so. Arrayjet will sell you an inkjet-based arrayer that can print 'proteins, cell lysates, carbohydrates, lectins, DNA & oligos': http://www.arrayjet.co.uk/products
This is not only practical, it's big business.
For a more accurate flowchart of Primer (and several other movies) see: http://xkcd.com/657/
In the old days we had a choice of shock therapy!
Slackware is still available!
Not a virologist, but your response doesn't sound kosher. I don't see anywhere in your references, or any scientific citation linked by anyone at this site, anything at all to suggest that Gene VI insertion was at all Intentional.
The point is that the 35S promoter and gene VI overlap in the viral genome, or to put it another way the same bit of sequence has multiple functions (this is common in viruses, which tend to make very efficient use of their genetic material).The sequence in question is at about 10 o'clock in this circular genome map:
http://en.wikipedia.org/wiki/File:CauliflowerMosaicRNA35S.png
The fine inner broken ring is the 35S transcript, and the promoter is at its 'blunt' end. This aligns with the 'sharp' end of gene VI ('TAV', in red).
If you use the promoter in a GM plant (as is commonly done) then you're inevitably also bringing along a fragment of the gene, since it's actually the same sequence. This is not the same thing as inserting the entire gene, of course, though the fragment may have some subset of the gene's multiple known functions. The DNA construct used to make the GM plants is not designed to express this fragment, but there's a possibility than once inserted in the host genome (depending on the surrounding sequence elements), the fragment could be expressed from some other promoter, making a protein that is eventually eaten by us.
"...(there's at least 2 strands of evidence for this)..."
I see what you did there.
An intermission would give me just enough time to think seriously about the horrible decision I've made and how hours of my life would be better spent by going home for a beer and a book.
May I suggest The Hobbit? At 1.4Mb (including the illustrations) the .epub would fit on a floppy and, I suspect, still end up saying more than Jackson's multi-petabyte trilogygasm.
"Why, I feel all thin, sort of stretched, if you know what I mean: like butter that has been scraped over too much bread." - Bilbo Baggins.
For people who shed tears because they don't like Win8 or think it needs "feature X" I have three letters for you:
SP1
You may need 4 letters:
http://www.theverge.com/2012/11/28/3693368/windows-blue-update-low-cost
Of course, using 4 letter words to describe Windows releases is quite common!
If there had been some EASY and OBVIOUS way to get rid of the METRO interface and go back to a Classic Shell she might have been happy with it, but after an hour of trying to do anything useful she wanted it boxed up and she has already returned it for a refund.
The Classic Shell I use is a third party addon:
http://classicshell.sourceforge.net/
It's a useful project that has been adding back features removed by MS from Vista onwards. Their start menu actually pre-dates Win 8 - it's more configurable than the standard Win 7 menu.
Of course, it's crazy that downloading something like this is even necessary!
Astonishing isn't it? They've taken an excellent product (by MS standards) and done their best to bury it under a silly hybrid UI setup. Take 10 minutes to install Classic Shell, configure it to boot straight to the desktop (start menu enabled, hot corners disabled), re-register the file types that have been hijacked by Metro apps, and you have arguably the best conventional version of Windows to date - fast booting, integrated antivirus, upgraded task manager, ISO mounting, and a nice clean theme, etc.
Basically all the bad press could have been avoided if they'd made Metro and the start menu globally optional without third party solutions. IT departments (even if they get past the reviews) will take one look at the default configuration and its unpredictable switches between desktop and Metro, think support calls, and file the whole thing as 'Do Not Want'. That MS are already making noises about Windows Blue for 2013 suggests they've realised there's little chance of widespread corporate adoption for Windows 8.
"We hate features and like to pretend that Gnome classic doesn't exist" - The Gnome 3 Roadmap (Status: Implementation in progress).
FTFY
Lastly, you dont need the start button if you learn how to use whats there.. Thats like complaining when going from Win 3.1 to 95. They got rid of my Program Manager I wish they gave me a way to turn it back on..
They did, from 95 up until XP SP1 ( http://support.microsoft.com/kb/142255 ). Of course back then they actually had people who thought about the impact of new interface design on users:
http://www.sigchi.org/chi96/proceedings/desbrief/Sullivan/kds_txt.htm
At one point in the design of Windows 95 they considered having two separate UIs, the windowed interface we know and a separate, simplified interface they thought might be suitable for beginners, and which seems to have featured a set of tiles that launched the various applications. Although the design "tested well, because it successfully constrained user actions to a very small set", it was abandoned because "If just one function a user needed was not supported in the beginner shell, s/he would have to abandon it (at least temporarily)", learning "would not necessarily transfer well to the standard shell", and "users had to learn two ways of interacting with the computer, which was confusing". I wonder if the Windows 8 design team were aware of this document..?
Maybe it's the airlines who are worried about the potential for violence inherent in being stuck next to someone jabbering away on their phone for an 8 hour flight? I hope they block Skype...
Though all my Nikon lenses are AF, I've always been tempted to get the classic 105mm f/2.5 AI (hey, maybe that'll make me shoot like Steve McCurry!). But an 85mm would actually make more sense on my D300 with the DX crop...
I have some of this gear too, including a Leica IIIa that's contemporary with the lens. As you probably know, forward compatibility isn't as good for the bodies (you can't just use an adapter and retain infinity focus with a bayonet lens), but there are a few modern screwmount lenses like those made by Cosina/Voigtlaender, so it's still possible to use pieces of kit made 70 years apart.
That's true, and though there were a few other (now obscure) still cameras that shot 24x36, 35mm as we know it effectively dates from the first production Leicas in the 1920s (which is still a pretty decent run for a tech standard). 'Half frame' (like the movie frame) still cameras were still made as late as the 80s, though, and some (like the original Olympus Pen) were quite popular.
I have a lens made for a 1930s Leica which, using an adapter they started making in the 50s (when the current bayonet mount was introduced), will work happily with any of their later rangefinder cameras, including the latest 2012 digital model (if I could afford it). As a bit of a long shot, I emailed the company a few years ago with a technical query about this lens, and got a prompt response with a request for the serial number so they could check their records! The standard flash/accessory shoe used today is also the same size as the one Leica was using as early as the 1920s, as is the 35mm cassette (so you can stick modern film in that antique Leica).
35mm itself (packaged differently) is basically a 19th century movie film standard, and we're also in the third century of several other common tech standards - the D cell battery goes back to 1898, the 1/4 inch audio jack is a 19th century phone switchboard plug, and the Edison screw lightbulb dates from the same era. Any others?
Most likely it's way too technical for today's /. average reader and editor.
...and probably because the conclusions of the paper have very little in common with the massively hyped version on medicaldaily.com. The original authors are much more cautious (and certainly don't claim that this is _the_ difference):
"Taken together, the unusual features of miR-941 evolution, as well as its potential association with functions linked to human longevity and cognition, suggest roles of miR-941 in the evolution of human-specific phenotypes."
A quick question; why can't I have both mate and cinnamon installed on the same system.
You probably can. A major reason for renaming everything in MATE was to allow co-existence with Gnome 3-based systems (which would include Cinnamon). You'll get one from the installation media, and can install the other from the repositories, then choose which you want to run at login time. However, each is a complete desktop (MATE rather more so) so you probably only need one.
30 years ago you didn't need an implant, you just had to think in Russian:
http://www.youtube.com/watch?v=S5DsLow4SVQ#t=1m21s
The problem is most any terrestrial network protocol expects a minimal signal-response delay between nodes
RFC 1149 does not assume this, though the current implementation would have to be modified to avoid complete packet loss in a non-terrestrial environment - BF Skinner's work suggests one obvious adaptation:
http://tools.ietf.org/html/rfc1149
http://historywired.si.edu/object.cfm?ID=353
i just tried it for the first time last night as a possible alternative to unity for new linux users but found problems and then saw why.
I've been using it for about a year, and don't see any major problems. It's my standard desktop on Ubuntu, and on the Mint VMs I have on Windows machines. It works about as well as any other desktop, including the old Gnome 2 - certainly well enough to be adopted by Mint and Fedora. It's faster and more complete than Cinnamon, which is a decent attempt at 'a gnome2 style interface with modern components'.
It should die just because you aren't interested? Mate does use gtk2 right now (just like Xfce, etc.) which is stable rather than unsupported, and continues to receive small updates and bug fixes. As for the other libraries, here's a comment from one of the developers:
http://bugs.debian.org/cgi-bin/bugreport.cgi?bug=658783#95
"MATE team is working hard to do this and we are in a good point. Migration to gsettings/dbus (and deletion of obsolete libs like bonobo, gconf, gnome-vfs, libgnome, libgnomeui and libgnomecanvas) is almost complete, and GNOME developers helped us on this too."
Those poor orchids, feeling the wrath of Darwin
"We found on St. Paul's only two kinds of birds - the booby and the noddy. The former is a species of gannet, and the latter a tern. Both are of a tame and stupid disposition, and are so unaccustomed to visitors, that I could have killed any number of them with my geological hammer."
- Charles Darwin, 'The Voyage of the Beagle'.