Presumably the mice are housed in BSL2-4 conditions. Especially if they are in a Level 3 or 4 lab, you can rest easy at night. Given proper attention by the animal handlers/workers who care for the mice, they won't be in the wild any time soon.
Perhaps all of us/.ers who are Molecular biologists should do a genomic DNA extraction tomorrow. Phenol/Chloroform.... keep it old school. Then resuspend in 40 uL TE then toss our Eppendorf tubes on the curb.
Of course he can make wild accusations unsupported by evidence.... this is Slashdot right?
And for a final kick in that poor fool's teeth, try explaining the enzymology of 4-stranded DNA replication.
While Franklin was not awarded a Nobel (dead by that time), M. Wilkins was. He was Franklin's boss of sorts, and was the person who did the actual "sharing" of data with Watson and Crick (or was foolish enough to allow them to steal it, or whomever you care to beleive).
Without actually having met Francis Crick and Jim Watson, I would have to say that Crick was motivated to only work with the 1st Bessel solution do to a physicists hope of elegance and simplicity.
I would like to hear your thoughts on base pairing in 4 stranded DNA. Surely this is a very large obstacle to overcome. There are other conformations of DNA in vivo, specifically Z-DNA, but for the most part B-DNA is where its at biologically. DNA is a fair bit rigid so there are not a great deal of reasonable conformations possible that would maintain the integrity of the molecule. Breaking bonds and re-ordering the molecule is a good way to introduce mutations. Not something that you want to have happen.
Discoveries from the structure of DNA.... how about the mechanism of replication, transcription, translation, mechanisms of mutagenesis, mechanisms of transcriptional regulation......
There is alot of "new hotness" in the field of bioinformatics. You could program (use skills you already have) to do biology(what you love). Just my $.02
The soccer team became self-aware at 6:21 pm. Slashdotters everywhere scared shitless. Knew the end was coming, but were more concerned about their kernels.
I wonder what would happen if my left hand wanted to know what my right hand was doing. Sure it may only be a few microvolts, but I wonder what my heart would think of the whole thing.
Check out all the sweet source code
on
Open Source Life?
·
· Score: 1
Here look. Open source genomes. Imagine that.
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi
The servers run *nix as well. Everyone happy now?
Take a look around, but please try and not/. this site, many of us rely on the ncbi for much of our daily research work. Using *nix. With open source utilities.
Did I mention *nix ?
Re:open source and patented are orthogonal
on
Open Source Life?
·
· Score: 1
PCR allows amplification of DNA, but for the most part requires that you know the sequence of about 20 nucleotides on each side of what you want to amplify (there are variations and exceptions.... I dont need to hear it unless you are willing to help redesign 1300 primers).
Current ddNTP (Sanger) method sequencing actually can be reasonably quick. And by the way, Sanger did win one of his two Nobels for dna suqencing (the other for protein sequencing).
" Were not very close to comparing what DNA a person has and saying, "Hey you'll be allergic to this drug" yet, "
Actually we are. A few hours of PCR can tell wether or not you metabolize a certain drug slowly, and thus need less, notably with heparin. Several polymorphisms have been characterized that prevent proper codein metabolism to morphine, essentially rendering codeine useless as an analgesic in such patients.
Continuing the precious metal thread... Bacterial resistance to, and metabolism of ionic mercury to the elemental form has been well known and characterized for many years. (search for mer operon if you want more)
I seem to remember an invention about a hundred or so years ago. It didn't put out as much light as the current gaslight technology, required a completely new , expensive, system to support it. But I guess the lightbulb worked out pretty well.
It is interesting to see Google data displayed this way. Probably most interesting simply because of how this visualization method has been already used for handling other extremely large data sets; DNA microarrays. Just take a look http://www.stanford.edu/group/cyert/
I too have noticed that, at least for now, Firefox takes a bit longer to open than IE. I dont even know if a user could really consider it an issue, as Firefox definately spawns new windows and tabs faster than IE, and oh yes.... its actually a useable browser.
I would hide the Ark of Cisco in the city of Tannis. Any network that carried this infront of it would be immune to getting/.'ed. But, you have to be careful for those network Nazis. Now, does anyone know where I could get a staff of RAM ?
After working in a lab for a few years, using SI units of course, I feel like a math cripple when someone asks me to do anything with English weights/volumes.
Example, how many gallons of water do I need to fill my 1600 cubic yard pool ?
All that power is so that Word will crash in the very beginning of your document, before you have really accomplished anything. If it crashes when theres nothing to loose, M$ figures people will stop bitchin'.
I must first preface this by saying I am a big physical security geek.
Many firesafes (especially the cheap ones)do not have an "endothermic reaction", but simply a water slurry in a liner between the outside and inside of the safe. If you remember your physics, specific heat of water is 4190J/kg K, and the heat of fusion is 330000J/kg or so. The vast majority of firesafes keep your documents cool and firefree by converting the water in their liners to steam, some of which does enter the inside of the safe in many cheap (think Sentry) models. Some firesafes have a tendency to be rather damp inside, so shopping around is a good idea.
And just to keep it on topic: All my usernames and passwords are kept in a sealed envelope in a safe that is kept in a seperate location from where I live. Sure a fire would toast it, but if I happen to die on the same night that a fire destroys those documents, well looks like everyone is SOL
They release it, two days later someone cracks it, writes a nice program to strip the DRM. Seems we've traveled down this road before. If it didn't work the first couple of times, why try again?
Slashdot Mirror
Dont you remember our physics class? The water would diffract the light, oh wait I remember, we spent our physics class programming calculators.
Presumably the mice are housed in BSL2-4 conditions. Especially if they are in a Level 3 or 4 lab, you can rest easy at night. Given proper attention by the animal handlers/workers who care for the mice, they won't be in the wild any time soon.
Perhaps all of us /.ers who are Molecular biologists should do a genomic DNA extraction tomorrow. Phenol/Chloroform.... keep it old school. Then resuspend in 40 uL TE then toss our Eppendorf tubes on the curb.
Of course he can make wild accusations unsupported by evidence.... this is Slashdot right? And for a final kick in that poor fool's teeth, try explaining the enzymology of 4-stranded DNA replication.
We kept on precipitating DNA and doing RNA preps... a bit of silent tribute.
While Franklin was not awarded a Nobel (dead by that time), M. Wilkins was. He was Franklin's boss of sorts, and was the person who did the actual "sharing" of data with Watson and Crick (or was foolish enough to allow them to steal it, or whomever you care to beleive).
Without actually having met Francis Crick and Jim Watson, I would have to say that Crick was motivated to only work with the 1st Bessel solution do to a physicists hope of elegance and simplicity. I would like to hear your thoughts on base pairing in 4 stranded DNA. Surely this is a very large obstacle to overcome. There are other conformations of DNA in vivo, specifically Z-DNA, but for the most part B-DNA is where its at biologically. DNA is a fair bit rigid so there are not a great deal of reasonable conformations possible that would maintain the integrity of the molecule. Breaking bonds and re-ordering the molecule is a good way to introduce mutations. Not something that you want to have happen. Discoveries from the structure of DNA.... how about the mechanism of replication, transcription, translation, mechanisms of mutagenesis, mechanisms of transcriptional regulation......
There is alot of "new hotness" in the field of bioinformatics. You could program (use skills you already have) to do biology(what you love). Just my $.02
Skynet....
The soccer team became self-aware at 6:21 pm. Slashdotters everywhere scared shitless. Knew the end was coming, but were more concerned about their kernels.
I wonder what would happen if my left hand wanted to know what my right hand was doing. Sure it may only be a few microvolts, but I wonder what my heart would think of the whole thing.
Here look. Open source genomes. Imagine that. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi The servers run *nix as well. Everyone happy now? Take a look around, but please try and not /. this site, many of us rely on the ncbi for much of our daily research work. Using *nix. With open source utilities.
Did I mention *nix ?
PCR allows amplification of DNA, but for the most part requires that you know the sequence of about 20 nucleotides on each side of what you want to amplify (there are variations and exceptions .... I dont need to hear it unless you are willing to help redesign 1300 primers).
Current ddNTP (Sanger) method sequencing actually can be reasonably quick. And by the way, Sanger did win one of his two Nobels for dna suqencing (the other for protein sequencing).
"
Were not very close to comparing what DNA a person has and saying, "Hey you'll be allergic to this drug" yet, "
Actually we are. A few hours of PCR can tell wether or not you metabolize a certain drug slowly, and thus need less, notably with heparin. Several polymorphisms have been characterized that prevent proper codein metabolism to morphine, essentially rendering codeine useless as an analgesic in such patients.
I will leave it up to you to do the research.
Continuing the precious metal thread... Bacterial resistance to, and metabolism of ionic mercury to the elemental form has been well known and characterized for many years. (search for mer operon if you want more)
I seem to remember an invention about a hundred or so years ago. It didn't put out as much light as the current gaslight technology, required a completely new , expensive, system to support it. But I guess the lightbulb worked out pretty well.
It is interesting to see Google data displayed this way. Probably most interesting simply because of how this visualization method has been already used for handling other extremely large data sets; DNA microarrays. Just take a look http://www.stanford.edu/group/cyert/
I too have noticed that, at least for now, Firefox takes a bit longer to open than IE. I dont even know if a user could really consider it an issue, as Firefox definately spawns new windows and tabs faster than IE, and oh yes.... its actually a useable browser.
"All your PhD are belong to us!"
/. discussion.
There, now we have a complete
I would hide the Ark of Cisco in the city of Tannis. Any network that carried this infront of it would be immune to getting /.'ed. But, you have to be careful for those network Nazis. Now, does anyone know where I could get a staff of RAM ?
After working in a lab for a few years, using SI units of course, I feel like a math cripple when someone asks me to do anything with English weights/volumes. Example, how many gallons of water do I need to fill my 1600 cubic yard pool ?
In Eastern PA where Im currently sitting, I can get gas at $1.82 a gallon, well at least I could yesterday, I havent been out yet today to see.
All that power is so that Word will crash in the very beginning of your document, before you have really accomplished anything. If it crashes when theres nothing to loose, M$ figures people will stop bitchin'.
I must first preface this by saying I am a big physical security geek.
Many firesafes (especially the cheap ones)do not have an "endothermic reaction", but simply a water slurry in a liner between the outside and inside of the safe. If you remember your physics, specific heat of water is 4190J/kg K, and the heat of fusion is 330000J/kg or so. The vast majority of firesafes keep your documents cool and firefree by converting the water in their liners to steam, some of which does enter the inside of the safe in many cheap (think Sentry) models. Some firesafes have a tendency to be rather damp inside, so shopping around is a good idea.
And just to keep it on topic: All my usernames and passwords are kept in a sealed envelope in a safe that is kept in a seperate location from where I live. Sure a fire would toast it, but if I happen to die on the same night that a fire destroys those documents, well looks like everyone is SOL
They release it, two days later someone cracks it, writes a nice program to strip the DRM. Seems we've traveled down this road before. If it didn't work the first couple of times, why try again?
With all those TFlops, no wonder Google converts units so quickly.