Obviously you are a terrible programmer if you look at it that way. I'm sure it took them all of an hour to "write the code", but surely much longer than that to come up with the algorithm.
swear Bush sounds more and more facist and like a smooth talking Hitler every day.
Hmm... I don't think GWB has ever been accused of being a "smooth talker" before. . .
Seriously... I have been getting a local college radio station on my TV when I try to watch simpsons reruns at 5:00 - 6:00 pm (CST). Fox is channel 6, here. I wonder if it is (was) related?
Just in case it get's slashdotted:
-------
BUFFALO, N.Y. -- Piero Bianco produces movies. Not films that chronicle the human condition, a la Hollywood. His subject is human biology at its most basic -- the translocation and unwinding of DNA by a DNA motor protein.
Bianco, an assistant professor in the Center for Single Molecule Biophysics and the Department of Microbiology, was the first to record on videotape in real time a molecule of a particular DNA motor protein in the process of "unzipping" a double strand of bacterial DNA.
To accomplish this feat, Bianco uses a novel technique he developed called "laser tweezers." Using this tool, he can grasp and hold a DNA molecule long enough to capture the action as the double helix unwinds.
"These laser tweezers allow us to look at one molecule at a time and understand how a protein really works," Bianco said. "When you look at groups of proteins, all the nuances of individual proteins are lost. With this system we can pull a DNA molecule out of a solution and actually watch a single DNA helicase molecule (the motor protein) take an individual DNA molecule and pull it apart."
If there were an Academy Award for most important basic science film, Bianco's work surely would be in the running. Knowing how DNA unwinds, copies and repairs itself -- what starts it, stops it and why -- will make possible major advancements in cancer treatment and is vitally important to the progress of gene therapy and recombinant DNA research.
Bianco currently is focusing his investigation and his camera on a motor protein more complex than the one captured on his first film and which has a different mode of action.
His goal at UB is to videotape in real time the actions of several motor proteins that accomplish DNA unwinding and DNA repair, and to use this knowledge to answer pressing questions about how cancer drugs work.
Since cancers are caused by uncontrolled cell growth, and DNA motor proteins make this possible by allowing DNA to copy itself, motor proteins are natural drug targets. Researchers know that many cancer drugs stop cell growth, but they don't know precisely how. Bianco is hoping to provide some details.
"We want to find out what happens when you put an anti-tumor drug in the way of the motor protein," Bianco said. "If the drug stops cell growth, we want to find out exactly how it does it. Will the protein start the unwinding with the drug present? If it starts, will it continue? Where will it stop, if at all?" The resulting movies will show how existing drugs work, and will allow researchers to test the efficacy of new drugs designed to inhibit DNA replication and repair.
Bianco developed his novel system while a postdoctoral fellow at the University of California at Davis, in collaboration with colleagues from Lawrence Livermore National Laboratory in Livermore, Calif. Creating it consumed several years, culminating in publication of the breakthrough in the journal Nature in January 2001.
Learning how a DNA motor protein functions and capturing it on film posed several vexing technical problems at the time: how to snatch a single DNA molecule, stretch it out, and hold it stable long enough to watch the helicase in action. Then there was the problem of size: DNA motor proteins, called helicases, are too small to be observed under a microscope.
Bianco solved these problems in a variety of ways. The laser tweezers form the crux of the system. By focusing an infrared laser beam through a microscope objective and aided by the laws of physics, he can create an optical trap that stops a DNA molecule in its tracks.
The molecule itself is too elusive to be caught, however, so Bianco tethers the DNA molecule to a microscopic polystyrene bead to give the tweezers something to grasp. Next he attaches a motor protein molecule to the opposite end of the DNA molecule, and tags both bead and DNA with a fluorescent dye. The dye creates an image sufficiently bright to be recorded by a micro-camera designed to perform under very-low-light conditions. Techniques weren't available earlier to bind fluorescent dye to the DNA helicase, but Bianco now has that capacity at UB.
Stretching out the molecule and initiating the action occurs in a flow cell -- a tiny, custom-made, Y-shaped apparatus the size of a microscope slide. Bianco introduces the bead and its cargo of DNA and motor protein into one channel of the flow cell (one of the arms of the "Y"), inserts ATP -- the molecular energy source -- into the other channel, and focuses an optical microscope and laser beam on the juncture of the channels.
The stretched-out molecule and its energy source flow in their separate channels into the juncture, where the action begins. The laser beam captures the polystyrene bead in its tweezers-like grip. Manipulating the laser beam, Bianco maneuvers the DNA into the path of the ATP, which jump-starts the helicase.
The breakthrough film described in Nature features a molecule of Escherichia coli helicase called RecBCD, which acts by unzipping the DNA molecule from one end to the other. That movie shows the stretched-out strands of glowing DNA becoming progressively shorter as the invisible motor protein unzips them, displacing the dye as it goes.
This action is called processive translocation. On film, it looks like a string of lights being switched off, one by one.
Once DNA strands are separated, the elemental processes of replication or repair can begin.
At UB, Bianco is adapting his novel system to investigate other, more complex bacterial helicases. The present target is RuvB, a circular nanomachine that drives a critical late step in genetic recombination called branch migration. Unlike RecBCD, this motor protein wraps itself around DNA like a doughnut on a string, carrying out translocation in a different manner. The UB set-up is equipped with two optical traps and is significantly more advanced than his former system, allowing him to work with complicated molecules such as RuvB.
The overarching goal of Bianco's research is to learn how cancer drugs interfere with translocation, which, in turn, will allow drug developers to target chemotherapy drugs to the most effective point in the process. He intends to define and film the action of several motor proteins, then begin working with four specific cancer drugs provided by collaborators at Roswell Park Cancer Institute.
"There is potential here," Bianco said, "to answer questions you could never answer any other way."
While entangle is useful for killing fleeing units (or peasants at mines), his Web Page Thorns aura is much better... Not to mention Web Page tranquility... but you have to creep slashdot a whole lot to get enough experience.
Slashdot Mirror
You're probably used to your own stink already, I don't see why it would be so hard. Really, doesn't your mom yell at you to clean up her basement?
EVERY TIME anyone has EVER geeked out about ANYTHING related to popular scifi/fantasy
Star Trek is popular now?
*grin*
*ducks*
I like this one.
They couldn't have, because evisa.com started up in 1997... a full 2 years prior to visa's trademark application.
Obviously you are a terrible programmer if you look at it that way. I'm sure it took them all of an hour to "write the code", but surely much longer than that to come up with the algorithm.
swear Bush sounds more and more facist and like a smooth talking Hitler every day. Hmm... I don't think GWB has ever been accused of being a "smooth talker" before. . .
Step one: Plug in. Step two: Get connected. Step three: There's no step three. THERE'S NO STEP THREE! Step four: ??? Step five: Profit!!
I've begun working with a PC-refurbishing operation which provides Win95 machines to folks in need. Does anyone have a solution to this problem?
I know it's hard, but just stop being a jackass and giving people win95 machines. If people can quit smoking, you can surely quit this atrocious act.
3) ??? 4) Profit
Wasn't having ants around enough? Now you are inviting crickets. . .
All I have to say is ew! Ew! Ew! Ew!!!
It's ok, you can get down off of the chair now, Daisy.
Seriously... I have been getting a local college radio station on my TV when I try to watch simpsons reruns at 5:00 - 6:00 pm (CST). Fox is channel 6, here. I wonder if it is (was) related?
I commend you, wise anonymous sir.
Whoops. Ladies and gentlemen, do you know what happened here? Repeat after me: Karma Whore Failure.
Sigh.
Just in case it get's slashdotted: ------- BUFFALO, N.Y. -- Piero Bianco produces movies. Not films that chronicle the human condition, a la Hollywood. His subject is human biology at its most basic -- the translocation and unwinding of DNA by a DNA motor protein. Bianco, an assistant professor in the Center for Single Molecule Biophysics and the Department of Microbiology, was the first to record on videotape in real time a molecule of a particular DNA motor protein in the process of "unzipping" a double strand of bacterial DNA. To accomplish this feat, Bianco uses a novel technique he developed called "laser tweezers." Using this tool, he can grasp and hold a DNA molecule long enough to capture the action as the double helix unwinds. "These laser tweezers allow us to look at one molecule at a time and understand how a protein really works," Bianco said. "When you look at groups of proteins, all the nuances of individual proteins are lost. With this system we can pull a DNA molecule out of a solution and actually watch a single DNA helicase molecule (the motor protein) take an individual DNA molecule and pull it apart." If there were an Academy Award for most important basic science film, Bianco's work surely would be in the running. Knowing how DNA unwinds, copies and repairs itself -- what starts it, stops it and why -- will make possible major advancements in cancer treatment and is vitally important to the progress of gene therapy and recombinant DNA research. Bianco currently is focusing his investigation and his camera on a motor protein more complex than the one captured on his first film and which has a different mode of action. His goal at UB is to videotape in real time the actions of several motor proteins that accomplish DNA unwinding and DNA repair, and to use this knowledge to answer pressing questions about how cancer drugs work. Since cancers are caused by uncontrolled cell growth, and DNA motor proteins make this possible by allowing DNA to copy itself, motor proteins are natural drug targets. Researchers know that many cancer drugs stop cell growth, but they don't know precisely how. Bianco is hoping to provide some details. "We want to find out what happens when you put an anti-tumor drug in the way of the motor protein," Bianco said. "If the drug stops cell growth, we want to find out exactly how it does it. Will the protein start the unwinding with the drug present? If it starts, will it continue? Where will it stop, if at all?" The resulting movies will show how existing drugs work, and will allow researchers to test the efficacy of new drugs designed to inhibit DNA replication and repair. Bianco developed his novel system while a postdoctoral fellow at the University of California at Davis, in collaboration with colleagues from Lawrence Livermore National Laboratory in Livermore, Calif. Creating it consumed several years, culminating in publication of the breakthrough in the journal Nature in January 2001. Learning how a DNA motor protein functions and capturing it on film posed several vexing technical problems at the time: how to snatch a single DNA molecule, stretch it out, and hold it stable long enough to watch the helicase in action. Then there was the problem of size: DNA motor proteins, called helicases, are too small to be observed under a microscope. Bianco solved these problems in a variety of ways. The laser tweezers form the crux of the system. By focusing an infrared laser beam through a microscope objective and aided by the laws of physics, he can create an optical trap that stops a DNA molecule in its tracks. The molecule itself is too elusive to be caught, however, so Bianco tethers the DNA molecule to a microscopic polystyrene bead to give the tweezers something to grasp. Next he attaches a motor protein molecule to the opposite end of the DNA molecule, and tags both bead and DNA with a fluorescent dye. The dye creates an image sufficiently bright to be recorded by a micro-camera designed to perform under very-low-light conditions. Techniques weren't available earlier to bind fluorescent dye to the DNA helicase, but Bianco now has that capacity at UB. Stretching out the molecule and initiating the action occurs in a flow cell -- a tiny, custom-made, Y-shaped apparatus the size of a microscope slide. Bianco introduces the bead and its cargo of DNA and motor protein into one channel of the flow cell (one of the arms of the "Y"), inserts ATP -- the molecular energy source -- into the other channel, and focuses an optical microscope and laser beam on the juncture of the channels. The stretched-out molecule and its energy source flow in their separate channels into the juncture, where the action begins. The laser beam captures the polystyrene bead in its tweezers-like grip. Manipulating the laser beam, Bianco maneuvers the DNA into the path of the ATP, which jump-starts the helicase. The breakthrough film described in Nature features a molecule of Escherichia coli helicase called RecBCD, which acts by unzipping the DNA molecule from one end to the other. That movie shows the stretched-out strands of glowing DNA becoming progressively shorter as the invisible motor protein unzips them, displacing the dye as it goes. This action is called processive translocation. On film, it looks like a string of lights being switched off, one by one. Once DNA strands are separated, the elemental processes of replication or repair can begin. At UB, Bianco is adapting his novel system to investigate other, more complex bacterial helicases. The present target is RuvB, a circular nanomachine that drives a critical late step in genetic recombination called branch migration. Unlike RecBCD, this motor protein wraps itself around DNA like a doughnut on a string, carrying out translocation in a different manner. The UB set-up is equipped with two optical traps and is significantly more advanced than his former system, allowing him to work with complicated molecules such as RuvB. The overarching goal of Bianco's research is to learn how cancer drugs interfere with translocation, which, in turn, will allow drug developers to target chemotherapy drugs to the most effective point in the process. He intends to define and film the action of several motor proteins, then begin working with four specific cancer drugs provided by collaborators at Roswell Park Cancer Institute. "There is potential here," Bianco said, "to answer questions you could never answer any other way."
While entangle is useful for killing fleeing units (or peasants at mines), his Web Page Thorns aura is much better... Not to mention Web Page tranquility... but you have to creep slashdot a whole lot to get enough experience.
Good luck having sex with even one lesbian.
You should be robbed of mod points if you mod down only in disagreement. The horror! The horror!
Give parent a life. Encourage parent to get out more. Encourage parent to destroy all video material of Carrot Top.
This business decision was denied by a Swarm of Microsoft Circus Mummies for BlueLight.com.
?
Unleash the hounds! The ones with bees in their mouths? Zoinks.
And then you undid it when you commented (even as an AC).
Am I missing something, or do you basically just have to use "alt" designators with your images, and not use flash? It doesn't seem that hard. . .
They should just release a "special" version for bank employees.
Is your tinfoil hat still securely in place?