I hear you. Ex-geek squad here. I was never able to look someone in the face and tell them yes, you really should pay us $29 to install that ram for you.
The most egregious markups are on the accessories. Look at the $25 USB printer cable or the $30 HDMI cable. They don't have to make the profit on the big ticket items if they can milk you on all the little things that go with them.
It was 6.x (don't remember the rest off-hand but can check later) on my work laptop. I was going to complain about it not existing but when I went to double check it was mysteriously there. Now at home on my desktop with 7.0.517.41 it's nowhere to be found.
Long story short, don't. It's not worth the risk. To "synthesize" your own you would need to obtain an isolated DNA sequence for insulin and transfect it into a cell line. Then culture the cell line and purify the insulin from cell products, most likely with some sort of chromatography. That said, this is not something you're going to easily accomplish at home. Producing proteins is not like making small molecule compounds. With small molecules you either have it or you don't. It isn't so cut and dry with proteins. Even a product of the same amino acid sequence can vary greatly in the post-translational modifications it undergoes. Prokaryotes don't glycosylate proteins and yeast hyperglycosylate is just one example. That's not to mention contamination from denatured protein and aggregates. Even if you did manage to create insulin, you would have to be crazy to think about injecting it into yourself. The purity is going to be dubious at best and you run the risk of developing an immune response to it. Worst case scenario, those antibodies are cross reactive to your body's endogenous insulin and you're now not only under-producing insulin, your body is attacking the little that you do make (or inject).
I have a friend with two misdemeanor DUIs--both of which are plead downs from DWIs. The second time he hit a parked car totaling his own vehicle. If he had one of these installed, it probably wouldn't have happened. Yes it sucks to have to pay for them after the first offense but for every person who blew a 0.1, there were probably several severely intoxicated drivers with good lawyers.
I've gotten one such email recently and another friend had 3 separate incidents from different contacts in the past month. It seems more probably than a massive increase it the success of brute force attacks.
that people are more stressed out by the perceived danger of illness after giving blood (and soon to give again) than by *pictures* of guns.
On a side note why on earth are they taking 10 mL of blood from patients when only 0.2 mL is being used in the assay?
thousands of legitimate concert-goers had to pay more for their tickets than they should have needed to
That assumes that these tickets wouldn't just have been bought by other scalpers before the actual even going public did. Certainly these guys weren't the only ones in the game, they just happened to be the quickest.
While proteins are among the most common causes of immune response, they are certainly not the only thing capable of invoking it. Carbohydrates and small molecule compoundscan elicit response as well. Look at common examples like nickel sulfate hexahydrate responsible for the allergic reaction to low purity gold or penicillin allergy.
When you order a computer with OS installation media, do those CD's / DVD's install the crapware as well, or just the basic OS?
Almost all of them have the crapware built in. I prefer to use OEM versions of windows discs (versus retail) and just input the key from the COA. You have to go pull drivers from the manufacturer's website but *usually* this is pretty painless.
University at Buffalo used to provide it's own customized version of Red Hat. It was the desktop environment that ran on all of the engineering lab computers. I think since they've discontinued it and just promote Ubuntu, but you can still access the technical documentation circa 2005.
Forcing NIH funded research to publish in specific journals would undermine the entire established hierarchy. Big name journals, large impact factor, etc...
The NIH public access policy currently requires any journal article resulting from funded research, regardless of the journal, to be made freely available.
Scientific papers are peer reviewed. The idea is by having other people look over the work and critique it, only papers meeting a certain standard are accepted. Any work published to a scientists own website has not undergone this review process and from the view of the scientific community is unvalidated.
As far as making money goes, the scientific community works a little differently. Scientists aren't looking for compensation from journals like Nature or Science. The goal of a scientists is to have their work cited. Bigger journals have a higher impact factor (the average # of times your article is cited). The more citations you have for your published works, the more funding you will get. The more funding you bring in, the more your institution will pay you (or better chance of tenure).
During undergrad as a major in math and pharmaceutics I really only saw limited exposure to Maple (Maple is free at my school so it's used instead of MatLab). Moving onto my PhD I took a class in FORTRAN. The goal was mostly to understand and appreciate the numerical methods that the packages we run simulations on utilize.
Until this thread I thought we were the only ones still using FORTRAN as our principle language. Misery loves company.
...are they fun? Actually they're surprisingly fun. It's taken up a couple hours of my time since reading this article yesterday.
On a side note there is one game called ESP where you and a partner are each shown a picture. You have to guess words until you each have guessed the same word. Often there will be "taboo" words that you can't use. Checkout this screenshot of the image and what gwap felt were necessary taboo words. Screenshot
That sounds about right. I'm doing my PhD now and have been in the same lab since the summer before my senior year of undergrad. Don't take it the wrong way but you simply don't (won't) have enough training to do any sort of real research. Other people will be reluctant to let you help with their projects by doing even some of the basics of their research because if things don't come out EXACTLY as expected, it means they have to redo it themselves to make sure there was nothing you messed up. Supervising you while you do the job just means it takes longer than doing it themselves.
I can just see it now "We won the lawsuit. We could give you the money or we could use that to sue twice as many people and you'll get twice the money in return. But wait, why stop there?"
Sounds like a Ponzi Scheme to me. Here's hoping the RIAA just takes all the money and disappears real soon.
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I hear you. Ex-geek squad here. I was never able to look someone in the face and tell them yes, you really should pay us $29 to install that ram for you.
The most egregious markups are on the accessories. Look at the $25 USB printer cable or the $30 HDMI cable. They don't have to make the profit on the big ticket items if they can milk you on all the little things that go with them.
It was 6.x (don't remember the rest off-hand but can check later) on my work laptop. I was going to complain about it not existing but when I went to double check it was mysteriously there. Now at home on my desktop with 7.0.517.41 it's nowhere to be found.
Chrome just got the print selection option (which I didn't realize was silently added until just now).
Long story short, don't. It's not worth the risk. To "synthesize" your own you would need to obtain an isolated DNA sequence for insulin and transfect it into a cell line. Then culture the cell line and purify the insulin from cell products, most likely with some sort of chromatography. That said, this is not something you're going to easily accomplish at home. Producing proteins is not like making small molecule compounds. With small molecules you either have it or you don't. It isn't so cut and dry with proteins. Even a product of the same amino acid sequence can vary greatly in the post-translational modifications it undergoes. Prokaryotes don't glycosylate proteins and yeast hyperglycosylate is just one example. That's not to mention contamination from denatured protein and aggregates. Even if you did manage to create insulin, you would have to be crazy to think about injecting it into yourself. The purity is going to be dubious at best and you run the risk of developing an immune response to it. Worst case scenario, those antibodies are cross reactive to your body's endogenous insulin and you're now not only under-producing insulin, your body is attacking the little that you do make (or inject).
I have a friend with two misdemeanor DUIs--both of which are plead downs from DWIs. The second time he hit a parked car totaling his own vehicle. If he had one of these installed, it probably wouldn't have happened. Yes it sucks to have to pay for them after the first offense but for every person who blew a 0.1, there were probably several severely intoxicated drivers with good lawyers.
I had similar problems with firefox on my older eeepc running eeebuntu with a 4gb ssd. Creating a loopback file helped a lot of the issues.
Mod parent funny.
I say "enhance" out loud whenever I click the zoom button on google maps.
I've gotten one such email recently and another friend had 3 separate incidents from different contacts in the past month. It seems more probably than a massive increase it the success of brute force attacks.
that people are more stressed out by the perceived danger of illness after giving blood (and soon to give again) than by *pictures* of guns. On a side note why on earth are they taking 10 mL of blood from patients when only 0.2 mL is being used in the assay?
thousands of legitimate concert-goers had to pay more for their tickets than they should have needed to
That assumes that these tickets wouldn't just have been bought by other scalpers before the actual even going public did. Certainly these guys weren't the only ones in the game, they just happened to be the quickest.
While proteins are among the most common causes of immune response, they are certainly not the only thing capable of invoking it. Carbohydrates and small molecule compoundscan elicit response as well. Look at common examples like nickel sulfate hexahydrate responsible for the allergic reaction to low purity gold or penicillin allergy.
When you order a computer with OS installation media, do those CD's / DVD's install the crapware as well, or just the basic OS?
Almost all of them have the crapware built in. I prefer to use OEM versions of windows discs (versus retail) and just input the key from the COA. You have to go pull drivers from the manufacturer's website but *usually* this is pretty painless.
University at Buffalo used to provide it's own customized version of Red Hat. It was the desktop environment that ran on all of the engineering lab computers. I think since they've discontinued it and just promote Ubuntu, but you can still access the technical documentation circa 2005.
I guess a one time fee of $0.99 isn't too much to ask. I do have over that with the change in my pocket from my two coffees I go this morning.
Any time I have $1.00 or more in change, I feel like I messed up somewhere along the way.
Forcing NIH funded research to publish in specific journals would undermine the entire established hierarchy. Big name journals, large impact factor, etc...
The NIH public access policy currently requires any journal article resulting from funded research, regardless of the journal, to be made freely available.
Solve the following math problem to continue:
1/0 = ?
Scientific papers are peer reviewed. The idea is by having other people look over the work and critique it, only papers meeting a certain standard are accepted. Any work published to a scientists own website has not undergone this review process and from the view of the scientific community is unvalidated.
As far as making money goes, the scientific community works a little differently. Scientists aren't looking for compensation from journals like Nature or Science. The goal of a scientists is to have their work cited. Bigger journals have a higher impact factor (the average # of times your article is cited). The more citations you have for your published works, the more funding you will get. The more funding you bring in, the more your institution will pay you (or better chance of tenure).
...they generally outsource all their hacking in exchange for WoW gold.
During undergrad as a major in math and pharmaceutics I really only saw limited exposure to Maple (Maple is free at my school so it's used instead of MatLab). Moving onto my PhD I took a class in FORTRAN. The goal was mostly to understand and appreciate the numerical methods that the packages we run simulations on utilize. Until this thread I thought we were the only ones still using FORTRAN as our principle language. Misery loves company.
...are they fun? Actually they're surprisingly fun. It's taken up a couple hours of my time since reading this article yesterday.On a side note there is one game called ESP where you and a partner are each shown a picture. You have to guess words until you each have guessed the same word. Often there will be "taboo" words that you can't use. Checkout this screenshot of the image and what gwap felt were necessary taboo words. Screenshot
Ah yes, the surface... http://youtube.com/watch?v=CZrr7AZ9nCY
That sounds about right. I'm doing my PhD now and have been in the same lab since the summer before my senior year of undergrad. Don't take it the wrong way but you simply don't (won't) have enough training to do any sort of real research. Other people will be reluctant to let you help with their projects by doing even some of the basics of their research because if things don't come out EXACTLY as expected, it means they have to redo it themselves to make sure there was nothing you messed up. Supervising you while you do the job just means it takes longer than doing it themselves.
I can just see it now "We won the lawsuit. We could give you the money or we could use that to sue twice as many people and you'll get twice the money in return. But wait, why stop there?"
Sounds like a Ponzi Scheme to me. Here's hoping the RIAA just takes all the money and disappears real soon.